Characterization of reactive oxidant species generated by neutrophils isolated from healthy and MPO-deficient subjects
| . | HOCl production (nmol/106 per mL/h) . | O2• − production (nmol/106 per mL/h) . |
|---|---|---|
| Healthy subjects | ||
| PMN (MPO+) | 29 ± 4.1 | 77 ± 14 |
| MPO-deficient subjects | ||
| PMN (MPO−) | 0.2 ± 0.2 | 125 ± 38 |
| . | HOCl production (nmol/106 per mL/h) . | O2• − production (nmol/106 per mL/h) . |
|---|---|---|
| Healthy subjects | ||
| PMN (MPO+) | 29 ± 4.1 | 77 ± 14 |
| MPO-deficient subjects | ||
| PMN (MPO−) | 0.2 ± 0.2 | 125 ± 38 |
Neutrophils (1 × 106/mL) isolated from healthy and MPO-deficient patients were incubated at 37°C in HBSS (pH 7.0) supplemented with 100 μM DTPA. Cells were activated by the addition of phorbol myristate acetate (200 nM), and cellular production of either HOCl or O2•− was determined as described under “Materials and methods.” Results represent the mean ± SD of neutrophil preparations isolated from 4 distinct subjects in each group.