dCK activities and IC50 concentrations in retrovirally transduced U937 cells
| Cells . | dCK activity . | IC50, μM . |
|---|---|---|
| U937 | 158 ± 0.006 | 2.97 ± 2.23 |
| U937/GFP | 135 ± 0.002 | 4.39 ± 2.70 |
| U937/wt | > 1000 | 2.42 ± 1.96 |
| U937/del 5 | 110 ± 0.002 | 4.05 ± 3.40 |
| U937/del 3-4 | 142 ± 0.008 | 2.94 ± 1.79 |
| U937/del 3-6 | 124 ± 0.000 | 2.69 ± 2.06 |
| Cells . | dCK activity . | IC50, μM . |
|---|---|---|
| U937 | 158 ± 0.006 | 2.97 ± 2.23 |
| U937/GFP | 135 ± 0.002 | 4.39 ± 2.70 |
| U937/wt | > 1000 | 2.42 ± 1.96 |
| U937/del 5 | 110 ± 0.002 | 4.05 ± 3.40 |
| U937/del 3-4 | 142 ± 0.008 | 2.94 ± 1.79 |
| U937/del 3-6 | 124 ± 0.000 | 2.69 ± 2.06 |
dCK activities (pmol/min × mg) were calculated from dCK phosphorylation assays using 0.01 mM 2-CdA as a substrate and were performed in duplicate experiments. IC50 concentrations for AraC were calculated from 3 independent WST-1 assays, each performed in triplicate experiments and presented as mean ± SD.