Absolute number of hematopoietic cells generated from 1000 EGFP-IRES-transduced or HOX-A10-IRES-EGFP-transduced CD34+cord blood progenitor cells after MS-5 stromal cell culture in the presence of various human cytokines
| . | IRES-EGFP . | HOX-A10-IRES-EGFP . |
|---|---|---|
| CD56+ NK cells | 3.7 × 105 (0.7-9.4 × 105) | 0.7 × 105 (0.1-1.2 × 105) |
| CD19+ B cells | 14.5 × 105 (4.9-35.1 × 105) | 1.6 × 105 (0.2-4.1 × 105) |
| CD14+ monocytic cells | 0.9 × 106 (0.6-1.6 × 106) | 6.0 × 106 (2.0-12.7 × 106) |
| CD15+ granulocytic cells | 2.5 × 106 (0.9-4.3 × 106) | 1.7 × 106 (0.5-3.6 × 106) |
| . | IRES-EGFP . | HOX-A10-IRES-EGFP . |
|---|---|---|
| CD56+ NK cells | 3.7 × 105 (0.7-9.4 × 105) | 0.7 × 105 (0.1-1.2 × 105) |
| CD19+ B cells | 14.5 × 105 (4.9-35.1 × 105) | 1.6 × 105 (0.2-4.1 × 105) |
| CD14+ monocytic cells | 0.9 × 106 (0.6-1.6 × 106) | 6.0 × 106 (2.0-12.7 × 106) |
| CD15+ granulocytic cells | 2.5 × 106 (0.9-4.3 × 106) | 1.7 × 106 (0.5-3.6 × 106) |
Values are the means (range) from 5 independent experiments with 5 different donors. Differences in cell numbers between IRES-EGFP-transduced cells and HOX-A10-IRES-EGFP-transduced cells were significant for all cell types (P < .05 on nonparametric paired Wilcoxon testing).
EGFP indicates enhanced green fluorescent protein; IRES, internal ribosome entry site; and NK, natural killer.