Primers used for PCR analysis
| Primer . | Sequence . |
|---|---|
| PCR Berlin | |
| RT-PCR primer | 5′-TGATTATAGCCTAAGACCCGGA-3′ (2o, ABL II) |
| 1. PCR round | 5′-TGATTATAGCCTAAGACCCGGA-3′ (2o, ABL II) |
| 5′-ATCTGCCTGAAGCTGGTGGGCT-3′ (1o1, ABL Ia) | |
| 5′-GCAGCAGCCTGGAAAAGTACTT-3′ (1o2, ABL Ib) | |
| 5′-GAAGTGTTTCAGAAGCTTCTCC-3′ (5o, BCR 2) | |
| 5′-ACCATCGTGGGCGTCCGCAAGA-3′ (7o, BCR I) | |
| 2. PCR round | 5′-ACTGAAGCCGCTCGTTGGAACTCCAA-3′ (1i, ABL II) |
| 5′-ATCTCCAGTGGCCAGAAAATCATACA-3′ (2i, ABL II) | |
| 5′-TGGAGCTGCAGATGCTGACCAACTCG-3′ (5i, BCR 2) | |
| 5′-AGATCTGGCCCAACGATGGCGAGGGC-3′ (7i, BCR I) | |
| PCR Heidelberg | |
| RT-PCR | Random hexamers |
| 1. PCR round | 5′-CAGCGGCCAGTAGCATCTGACTTTG-3′ (3o, ABL II) |
| 5′-CCATTTTTGGTTTGGGCTTCACACCATTCC-3′ (4o, ABL III) | |
| 5′-GACGCGTGCAGAGTGGAGGGAGAACATCCGG-3′ (6o, BCR 1) | |
| 5′-ATGGCGAGGGCGCCTTCCAT-3′ (8o/i, BCR I) | |
| 2. PCR round | 5′-GCCTCAGGGTCTGAGTGAAGCCGCTCGTTG-3′ (3i, ABL II) |
| 5′-TGTGATTATAGCCTAAGACCCGGAGCTTTTC-3′ (4i, ABL III) | |
| 5′-GAAGAAGTGTTTCAGAAGCTTCTCC-3′ (6i, BCR 2) | |
| 5′-ATGGCGAGGGCGCCTTCCAT-3′ (8o/i, BCR I) |
| Primer . | Sequence . |
|---|---|
| PCR Berlin | |
| RT-PCR primer | 5′-TGATTATAGCCTAAGACCCGGA-3′ (2o, ABL II) |
| 1. PCR round | 5′-TGATTATAGCCTAAGACCCGGA-3′ (2o, ABL II) |
| 5′-ATCTGCCTGAAGCTGGTGGGCT-3′ (1o1, ABL Ia) | |
| 5′-GCAGCAGCCTGGAAAAGTACTT-3′ (1o2, ABL Ib) | |
| 5′-GAAGTGTTTCAGAAGCTTCTCC-3′ (5o, BCR 2) | |
| 5′-ACCATCGTGGGCGTCCGCAAGA-3′ (7o, BCR I) | |
| 2. PCR round | 5′-ACTGAAGCCGCTCGTTGGAACTCCAA-3′ (1i, ABL II) |
| 5′-ATCTCCAGTGGCCAGAAAATCATACA-3′ (2i, ABL II) | |
| 5′-TGGAGCTGCAGATGCTGACCAACTCG-3′ (5i, BCR 2) | |
| 5′-AGATCTGGCCCAACGATGGCGAGGGC-3′ (7i, BCR I) | |
| PCR Heidelberg | |
| RT-PCR | Random hexamers |
| 1. PCR round | 5′-CAGCGGCCAGTAGCATCTGACTTTG-3′ (3o, ABL II) |
| 5′-CCATTTTTGGTTTGGGCTTCACACCATTCC-3′ (4o, ABL III) | |
| 5′-GACGCGTGCAGAGTGGAGGGAGAACATCCGG-3′ (6o, BCR 1) | |
| 5′-ATGGCGAGGGCGCCTTCCAT-3′ (8o/i, BCR I) | |
| 2. PCR round | 5′-GCCTCAGGGTCTGAGTGAAGCCGCTCGTTG-3′ (3i, ABL II) |
| 5′-TGTGATTATAGCCTAAGACCCGGAGCTTTTC-3′ (4i, ABL III) | |
| 5′-GAAGAAGTGTTTCAGAAGCTTCTCC-3′ (6i, BCR 2) | |
| 5′-ATGGCGAGGGCGCCTTCCAT-3′ (8o/i, BCR I) |
o indicates outer primer; i, inner primer.