C1498/B7-1 and C1498/B7-2 induce tumor-specific CD8+ CTL in vitro
| Days in culture* . | Day 0 . | Day 5 . | Day 15 . | ||||
|---|---|---|---|---|---|---|---|
| Stimulator† . | — . | C1498 . | C1498/B7-1 . | C1498/B7-2 . | C1498 . | C1498/B7-1 . | C1498/B7-2 . |
| %CD4+ | 16.6 | 32.2 | 8.2 | 9.4 | 26.1 | 1.8 | 2.3 |
| %CD8+ | 11.4 | 20.7 | 31.5 | 40.2 | 53.5 | 88.0 | 85.3 |
| LU30 vs C1498‡ | 0.0 | 0.0 | 248.8 | 60.9 | 52.3 | 642.0 | 501.3 |
| LU30 vs FBL-3 | 0.0 | 0.0 | 92.9 | 17.4 | 0.02 | 18.1 | 11.6 |
| Days in culture* . | Day 0 . | Day 5 . | Day 15 . | ||||
|---|---|---|---|---|---|---|---|
| Stimulator† . | — . | C1498 . | C1498/B7-1 . | C1498/B7-2 . | C1498 . | C1498/B7-1 . | C1498/B7-2 . |
| %CD4+ | 16.6 | 32.2 | 8.2 | 9.4 | 26.1 | 1.8 | 2.3 |
| %CD8+ | 11.4 | 20.7 | 31.5 | 40.2 | 53.5 | 88.0 | 85.3 |
| LU30 vs C1498‡ | 0.0 | 0.0 | 248.8 | 60.9 | 52.3 | 642.0 | 501.3 |
| LU30 vs FBL-3 | 0.0 | 0.0 | 92.9 | 17.4 | 0.02 | 18.1 | 11.6 |
Naive splenocytes were cocultured with irradiated C1498, C1498/B7-1, or C1498/B7-2 stimulator cells in MLTR cultures. Every 5 days, half the contents of the culture wells was harvested for phenotypic analysis and lytic assays.
Cells remaining in the culture wells were restimulated with 2 × 104 irradiated C1498, C1498/B7-1, or C1498/B7-2 stimulator cells.
LU30 = number of lytic units per 1 × 106 cells. Data are representative of 3 separate experiments.