Table 2.

Migration of CD34+ cells expressing low and high levels of VLA-5

Cell fraction
VLA-5lowVLA-5high
% S/G2 + M cells 34.6 ± 1.0 62.0 ± 1.9  
% migration   
 Fn 38.7 ± 1.7* 20.0 ± 3.4 
 BSA 24.1 ± 3.4 26.2 ± 1.3 
Cell fraction
VLA-5lowVLA-5high
% S/G2 + M cells 34.6 ± 1.0 62.0 ± 1.9  
% migration   
 Fn 38.7 ± 1.7* 20.0 ± 3.4 
 BSA 24.1 ± 3.4 26.2 ± 1.3 

Cultured CD34+ cells were sorted into VLA-5low and VLA-5high fractions. The percent cells in S/G2 + M was determined in each fraction by PI staining. The migration capacity was assessed toward MS-5 CM across Fn (n = 6) or BSA (n = 2).

*

P < .05 compared with migration of VLA-5high cells across Fn.

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