Reversal of 5-HT actions by SSRI
| Treatments* . | % Viable cells† (24 h) . | % Caspase activation‡ (6 h) . |
|---|---|---|
| Control | 69.3 ± 1.20 | 27.3 ± 1.20 |
| 5-HT | 21.3 ± 8.65 | 58.3 ± 2.03 |
| Fluoxetine + 5-HT | 54.7 ± 0.88 | 30.3 ± 2.91 |
| Citalopram + 5-HT | 55.3 ± 4.98 | 29.3 ± 1.33 |
| Paroxetine + 5-HT | 58.0 ± 3.06 | 29.3 ± 4.98 |
| Treatments* . | % Viable cells† (24 h) . | % Caspase activation‡ (6 h) . |
|---|---|---|
| Control | 69.3 ± 1.20 | 27.3 ± 1.20 |
| 5-HT | 21.3 ± 8.65 | 58.3 ± 2.03 |
| Fluoxetine + 5-HT | 54.7 ± 0.88 | 30.3 ± 2.91 |
| Citalopram + 5-HT | 55.3 ± 4.98 | 29.3 ± 1.33 |
| Paroxetine + 5-HT | 58.0 ± 3.06 | 29.3 ± 4.98 |
L3055 cells were cultured with or without 5-HT (150 μM), as indicated. SSRI: fluoxetine (5 μM), citalopram (10 μM), paroxetine (2 μM).
At the end of a 24-hour culture period with treatments shown, cells were analyzed by FACS for forward versus side light scatter properties, as in Figure 2. Percentage viable cells remaining are given as means ± SEM of 3 experiments.
After 6-hour treatment, L3055 cells were stained with FAM-VAD-FMK and analyzed for active caspases as for Figure 3. Results are given as percentage cells active caspase positive and are represented as means ± SEM of 3 experiments.