Effect of H-ferritin on expression of DC markers
| Donor no. . | Treatment . | CD1a* % cells (MFI) . | CD80 % cells (MFI) . | CD83 % cells (MFI) . | CD86,† % cells (MFI) . | B7-H1,† % cells (MFI) . | HLA-DR % cells (MFI) . |
|---|---|---|---|---|---|---|---|
| Donor 1 | DCs | 60.0 (4.7) | 77.4 (7.4) | 30.9 (1.7) | 77.3 (7.0) | 96.0 (18.3) | 94.2 (26.2) |
| DCs + L-ferritin | 60.7 (4.1) | 77.2 (7.1) | 29.0 (1.6) | 75.1 (5.8) | 97.6 (23.7) | 95.6 (19.3) | |
| DCs + H-ferritin | 58.1 (4.1) | 90.3 (24.4) | 58.1 (3.7) | 94.5 (37.8) | 99.5 (114.5) | 96.2 (32.5) | |
| DCs + CD40L | 60.2 (4.9) | 79.1 (10.1) | 53.3 (3.3) | 91.0 (30.0) | 99.4 (53.8) | 95.5 (52.3) | |
| Donor 2 | DCs | 34.6 (1.6) | 37.7 (1.6) | 31.4 (1.3) | 97.2 (10.9) | 71.8 (2.8) | 89.6 (5.7) |
| DCs + L-ferritin | 33.3 (1.5) | 33.3 (1.6) | 32.2 (1.4) | 95.5 (8.1) | 70.7 (3.0) | 83.6 (5.0) | |
| DCs + H-ferritin | 28.0 (1.4) | 35.3 (1.5) | 37.6 (1.6) | 99.1 (69.2) | 85.2 (4.7) | 93.6 (17.9) | |
| DCs + CD40L | 31.4 (1.6) | 28.7 (1.4) | 33.2 (1.7) | 93.3 (21.7) | 72.7 (3.3) | 89.8 (15.1) | |
| Donor 3 | DCs | NA | 68.4 (3.8) | NA | 65.6 (2.9) | 78.0 (5.8) | 79.9 (10.9) |
| DCs + L-ferritin | NA | 69.4 (4.0) | NA | 63.7 (2.4) | 93.1 (10.3) | 81.1 (8.0) | |
| DCs + H-ferritin | NA | 83.4 (6.2) | NA | 80.1 (15.7) | 95.9 (23.9) | 81.6 (13.5) | |
| DCs + CD40L | NA | 68.8 (4.8) | NA | 77.2 (12.5) | 92.8 (18.4) | 81.0 (21.7) |
| Donor no. . | Treatment . | CD1a* % cells (MFI) . | CD80 % cells (MFI) . | CD83 % cells (MFI) . | CD86,† % cells (MFI) . | B7-H1,† % cells (MFI) . | HLA-DR % cells (MFI) . |
|---|---|---|---|---|---|---|---|
| Donor 1 | DCs | 60.0 (4.7) | 77.4 (7.4) | 30.9 (1.7) | 77.3 (7.0) | 96.0 (18.3) | 94.2 (26.2) |
| DCs + L-ferritin | 60.7 (4.1) | 77.2 (7.1) | 29.0 (1.6) | 75.1 (5.8) | 97.6 (23.7) | 95.6 (19.3) | |
| DCs + H-ferritin | 58.1 (4.1) | 90.3 (24.4) | 58.1 (3.7) | 94.5 (37.8) | 99.5 (114.5) | 96.2 (32.5) | |
| DCs + CD40L | 60.2 (4.9) | 79.1 (10.1) | 53.3 (3.3) | 91.0 (30.0) | 99.4 (53.8) | 95.5 (52.3) | |
| Donor 2 | DCs | 34.6 (1.6) | 37.7 (1.6) | 31.4 (1.3) | 97.2 (10.9) | 71.8 (2.8) | 89.6 (5.7) |
| DCs + L-ferritin | 33.3 (1.5) | 33.3 (1.6) | 32.2 (1.4) | 95.5 (8.1) | 70.7 (3.0) | 83.6 (5.0) | |
| DCs + H-ferritin | 28.0 (1.4) | 35.3 (1.5) | 37.6 (1.6) | 99.1 (69.2) | 85.2 (4.7) | 93.6 (17.9) | |
| DCs + CD40L | 31.4 (1.6) | 28.7 (1.4) | 33.2 (1.7) | 93.3 (21.7) | 72.7 (3.3) | 89.8 (15.1) | |
| Donor 3 | DCs | NA | 68.4 (3.8) | NA | 65.6 (2.9) | 78.0 (5.8) | 79.9 (10.9) |
| DCs + L-ferritin | NA | 69.4 (4.0) | NA | 63.7 (2.4) | 93.1 (10.3) | 81.1 (8.0) | |
| DCs + H-ferritin | NA | 83.4 (6.2) | NA | 80.1 (15.7) | 95.9 (23.9) | 81.6 (13.5) | |
| DCs + CD40L | NA | 68.8 (4.8) | NA | 77.2 (12.5) | 92.8 (18.4) | 81.0 (21.7) |
DCs were incubated with H- or L-ferritin or CD40L at a concentration of 1 μg/mL for 24 hours prior to detection of markers. MFI indicates median fluorescence intensity; NA, not available.
Measured by flow cytometry as % cells stained.
H-ferritin significantly increased CD86 (paired F test,P = .021) and B7-H1 (P = .019).