Effects of cycloheximide on stimulus-induced hepatocyte growth factor secretion
| . | Without CHX pretreatment . | With CHX pretreatment . |
|---|---|---|
| Control PMNs | 846 ± 134 | 897 ± 189 |
| LPS (1 μg/mL) | 1 480 ± 239 | 1 239 ± 192 |
| PMA (100 ng/mL) | 1 621 ± 212 | 1 408 ± 285 |
| fMLP (10−6 M) | 1 347 ± 540 | 1 524 ± 746 |
| IL-1 (10 ng/mL) | 1 068 ± 272 | 1 128 ± 360 |
| . | Without CHX pretreatment . | With CHX pretreatment . |
|---|---|---|
| Control PMNs | 846 ± 134 | 897 ± 189 |
| LPS (1 μg/mL) | 1 480 ± 239 | 1 239 ± 192 |
| PMA (100 ng/mL) | 1 621 ± 212 | 1 408 ± 285 |
| fMLP (10−6 M) | 1 347 ± 540 | 1 524 ± 746 |
| IL-1 (10 ng/mL) | 1 068 ± 272 | 1 128 ± 360 |
Polymorphonuclear neutrophils (PMN; 107/mL) were preincubated with or without cycloheximide (CHX) and were then stimulated for 24 hours at 37°C with various agents at the concentrations indicated; unstimulated control PMNs were incubated in medium alone. Hepatocyte growth factor (HGF) was assayed in the cell-free supernatants. Results are expressed as the mean ± SEM of HGF levels (pg/mL) in 3 experiments. LPS indicates lipopolysaccharide; PMA, phorbol myristate acetate; fMLP, N-formylmethionyl-leucyl-phenylalanine; IL-1, interleukin 1.