Thrombin-stimulated secretion of lysosomal enzymes from platelets of normal C3H/HeSnJ +/+ and C3H/HeSnJ ash/ashmutant mice
| . | Secretion (% total) . | |
|---|---|---|
| − Thrombin . | + Thrombin . | |
| C3H/HeSnJ +/+ (6) | ||
| β-glucuronidase | 1.82 ± 0.36 | 24.8 ± 1.8 |
| β-galactosidase | 1.34 ± 0.19 | 25.6 ± 1.9 |
| C3H/HeSnJ ash/ash(6) | ||
| β-glucuronidase | 3.60 ± 0.99 | 30.4 ± 1.9 |
| β-galactosidase | 1.56 ± 0.40 | 28.7 ± 1.0 |
| . | Secretion (% total) . | |
|---|---|---|
| − Thrombin . | + Thrombin . | |
| C3H/HeSnJ +/+ (6) | ||
| β-glucuronidase | 1.82 ± 0.36 | 24.8 ± 1.8 |
| β-galactosidase | 1.34 ± 0.19 | 25.6 ± 1.9 |
| C3H/HeSnJ ash/ash(6) | ||
| β-glucuronidase | 3.60 ± 0.99 | 30.4 ± 1.9 |
| β-galactosidase | 1.56 ± 0.40 | 28.7 ± 1.0 |
Platelets (1 × 109/mL) were treated with buffer or with 0.625 U/mL thrombin for 3 minutes at 37°C. The reaction was terminated with Thromstop. Values represent the mean ± SEM of the individual determinations on the number of mice indicated in parentheses.