Quantification of htal-1 messenger RNA levels in different animal lines
| Animal lines . | Meanhtal-1 mRNA . | Mean hprt mRNA . | Meantal-1/hprt ratio . |
|---|---|---|---|
| 10 copy L6 | 1.137 × 10−1 | 1.065 × 10−1 | 1.068 |
| 2 copy L8 | 8.144 × 10−3 | 4.572 × 10−2 | 0.178 |
| Wild type | 0 | 4.943 × 10−4 | 0 |
| Animal lines . | Meanhtal-1 mRNA . | Mean hprt mRNA . | Meantal-1/hprt ratio . |
|---|---|---|---|
| 10 copy L6 | 1.137 × 10−1 | 1.065 × 10−1 | 1.068 |
| 2 copy L8 | 8.144 × 10−3 | 4.572 × 10−2 | 0.178 |
| Wild type | 0 | 4.943 × 10−4 | 0 |
Real-time reverse transcriptase–polymerase chain reaction using Lightcycler technology (Roche) of unsorted bone marrow cells of L6, L8, and wild-type animal lines was performed to show correlation between transgene copy number and messenger RNA (mRNA) expression.Tal-1 mRNA was quantified relative to hprt mRNA levels. All samples were run in duplicate, and the meantal-1/hprt ratio of 2 experiments is shown.