RT-PCR and FISH analysis of sorted CD34+CD19− and CD34+CD19+ cell populations
| Patient no. . | Level of TEL/AML1-positive cells by RT-PCR . | Level of t(12;21)-positive cells by FISH . | |||
|---|---|---|---|---|---|
| Sorted cell populations . | Control cell lines . | ||||
| CD34+CD19−, % n = 8 . | CD34+CD19−, % n = 5 . | CD34+CD19+, % n = 6 . | BLIN-1 negative control, % n = 4 . | REH positive control, % n = 6 . | |
| 807/00 | 6.0 | 8.8 | 7.5* | 2.0 | 98.5 |
| 1523/00 | 0.1 | 12.0 | 96.0 | — | 98.0 |
| 1007/99 | 3.1 | — | 87.4 | 3.2 | 99.0 |
| 917/98 | 3.3 | — | — | — | — |
| 1318/97 | — | 1.0 | 94.5 | 3.3 | 98.6 |
| 658/97 | 0.0 | 5.4 | 96.0 | 4.0 | 92.0 |
| 589/97 | 0.0 | 8.9 | 99.2 | — | 98.0 |
| 008/B | 0.0 | — | — | — | — |
| 009/B | 0.6 | — | — | — | — |
| Median | 0.4 | 8.8 | 95.3 | 3.3 | 98.3 |
| Mean | 1.6 | 7.2 | 80.1 | 3.1 | 97.4 |
| SD | ± 2.2 | ± 4.2 | ± 35.8 | ± 0.8 | ± 2.6 |
| Patient no. . | Level of TEL/AML1-positive cells by RT-PCR . | Level of t(12;21)-positive cells by FISH . | |||
|---|---|---|---|---|---|
| Sorted cell populations . | Control cell lines . | ||||
| CD34+CD19−, % n = 8 . | CD34+CD19−, % n = 5 . | CD34+CD19+, % n = 6 . | BLIN-1 negative control, % n = 4 . | REH positive control, % n = 6 . | |
| 807/00 | 6.0 | 8.8 | 7.5* | 2.0 | 98.5 |
| 1523/00 | 0.1 | 12.0 | 96.0 | — | 98.0 |
| 1007/99 | 3.1 | — | 87.4 | 3.2 | 99.0 |
| 917/98 | 3.3 | — | — | — | — |
| 1318/97 | — | 1.0 | 94.5 | 3.3 | 98.6 |
| 658/97 | 0.0 | 5.4 | 96.0 | 4.0 | 92.0 |
| 589/97 | 0.0 | 8.9 | 99.2 | — | 98.0 |
| 008/B | 0.0 | — | — | — | — |
| 009/B | 0.6 | — | — | — | — |
| Median | 0.4 | 8.8 | 95.3 | 3.3 | 98.3 |
| Mean | 1.6 | 7.2 | 80.1 | 3.1 | 97.4 |
| SD | ± 2.2 | ± 4.2 | ± 35.8 | ± 0.8 | ± 2.6 |
The leukemic cells from patient no. 807/00 wereTEL/AML1-positive by RT-PCR.