The effects of the chelators on [3H]thymidine incorporation into SK-N-MC neuroepithelioma cells
| Chelator . | IC50, μM . |
|---|---|
| DFO | 23 ± 4.6 |
| 311 | 0.6 ± 0.1 |
| 311m | 0.6 ± 0.1 |
| ST | > 25 |
| NT | 9 ± 2.3 |
| N2mT | 15.5 ± 3.4 |
| N4mT | 6.3 ± 2.0 |
| N44mT | 1.5 ± 0.4 |
| N4eT | 5.7 ± 1.8 |
| N4aT | 7.9 ± 1.4 |
| N4pT | 3.8 ± 2.4 |
| N44pH | 4.3 ± 1.6 |
| NoctH | 17.4 ± 6.2 |
| FeNT | > 25 |
| Chelator . | IC50, μM . |
|---|---|
| DFO | 23 ± 4.6 |
| 311 | 0.6 ± 0.1 |
| 311m | 0.6 ± 0.1 |
| ST | > 25 |
| NT | 9 ± 2.3 |
| N2mT | 15.5 ± 3.4 |
| N4mT | 6.3 ± 2.0 |
| N44mT | 1.5 ± 0.4 |
| N4eT | 5.7 ± 1.8 |
| N4aT | 7.9 ± 1.4 |
| N4pT | 3.8 ± 2.4 |
| N44pH | 4.3 ± 1.6 |
| NoctH | 17.4 ± 6.2 |
| FeNT | > 25 |
The chelators and the iron complex of NT were incubated with cells for 20 hours. At the end of this incubation period, [3H]thymidine was added and the cells were reincubated for 2 hours. Incorporation of [3H]thymidine was determined as described in “Materials and methods.” Results are the mean ± SD (3 experiments).
IC50 indicates 50% inhibitory concentration; DFO, desferrioxamine; other abbreviations are explained in the legend of Figure 1.