Detection of TCR-γ rearrangements in single CD30+ as well as in single CD30− cells
| Patient no. . | CD30+cells . | CD30− cells . | |||
|---|---|---|---|---|---|
| No. of isolated cells . | Sequences identical/unrelated3-150 . | Vγ family . | No. of isolated cells . | Sequences identical3-151/unrelated3-150 . | |
| 1A3-152 | 34 | 6;53-153/3 | V4/V4 | 18 | 1/7 |
| 1B3-152 | 36 | 11;63-153/0 | V4/V4 | 16 | 0/3 |
| 2C3-152 | 24 | 10;73-153/0 | V4/V8 | 123-155 | 0/5 |
| 2D3-152 | 18 | 5;73-153/0 | V4/V8 | 183-155 | 1/16 |
| 3E3-152 | 34 | 11/0 | V4 | 163-155 | 0/3 |
| 3F3-152 | 18 | 5/0 | V4 | 243-155 | 0/2 |
| 4 | 36 | 15/0 | V2 | 14 | 0/6 |
| 5 | 36 | 8/2 | V5 | 163-155 | 0/9 |
| 6 | 36 | 5;43-153/0 | V3/V8 | 183-155 | 1/10 |
| 7 | 24 | 3/0 | V8 | 183-155 | 1/7 |
| 8 | 34 | 7/0 | V10 | 163-155 | 0/4 |
| 9 | 13 | 3/0 | V9 | 163-155 | 0/3 |
| 10 | 26 | 0/0 | 3-154 | 73-155 | 0/0 |
| 11 | 18 | 0/0 | 3-154 | 0 | — |
| Total | 387 | 118/5 | 209 | 4/75 | |
| Patient no. . | CD30+cells . | CD30− cells . | |||
|---|---|---|---|---|---|
| No. of isolated cells . | Sequences identical/unrelated3-150 . | Vγ family . | No. of isolated cells . | Sequences identical3-151/unrelated3-150 . | |
| 1A3-152 | 34 | 6;53-153/3 | V4/V4 | 18 | 1/7 |
| 1B3-152 | 36 | 11;63-153/0 | V4/V4 | 16 | 0/3 |
| 2C3-152 | 24 | 10;73-153/0 | V4/V8 | 123-155 | 0/5 |
| 2D3-152 | 18 | 5;73-153/0 | V4/V8 | 183-155 | 1/16 |
| 3E3-152 | 34 | 11/0 | V4 | 163-155 | 0/3 |
| 3F3-152 | 18 | 5/0 | V4 | 243-155 | 0/2 |
| 4 | 36 | 15/0 | V2 | 14 | 0/6 |
| 5 | 36 | 8/2 | V5 | 163-155 | 0/9 |
| 6 | 36 | 5;43-153/0 | V3/V8 | 183-155 | 1/10 |
| 7 | 24 | 3/0 | V8 | 183-155 | 1/7 |
| 8 | 34 | 7/0 | V10 | 163-155 | 0/4 |
| 9 | 13 | 3/0 | V9 | 163-155 | 0/3 |
| 10 | 26 | 0/0 | 3-154 | 73-155 | 0/0 |
| 11 | 18 | 0/0 | 3-154 | 0 | — |
| Total | 387 | 118/5 | 209 | 4/75 | |
Unique sequence different from the clonal CD30+ cells.
Identical to clonal CD30+ cells.
Interval between the biopsies: A-B: 8 months; C-D: 52 months; E-F: 3 months.
Biallelic rearrangement.
Results from 2 to 6 pooled CD30− cells.
No amplificate by single-cell analysis, but dominant TCR-γ gene rearrangement demonstrated by GeneScan analysis.