Table 1. HOXB4 enhances production of B... | American Society of Hematology

Table 1.

HOXB4 enhances production of B cells in vitro

		Total CD19⁺ B cells GFP (× 10³)	Total CD19⁺ B cells B4 (× 10³)	Total CD34⁺CD19⁺ B-cell precursors GFP (× 10³)	Total CD34⁺CD19⁺ B-cell precursors B4 (× 10³)
3 GFs	3 wk	55.5 ± 39	94 ± 45	0.09 ± 0.09	3 ± 0.5
		(9.5 ± 6.4%)^*	(31.3 ± 15.1%)^*
	6 wk	1.6 ± 0.2	31.2 ± 3.5^†	0	0.5 ± 0.5^†
		(0.7 ± 0.4%)^*	(11.3 ± 2.2%)^*
6 GFs	3 wk	8.6 ± 7.2	67.8 ± 13.4^†	0.2 ± 0.06	4 ± 0.09^†
		(0.2 ± 0.1%)^*	(1.4 ± 0.2%)^*
	6 wk	1.6 ± 0.7	4.3 ± 0.1^†	0	0
		(0.1 ± 0.03%)^*	(0.3 ± 0.01%)^*

		Total CD19⁺ B cells GFP (× 10³)	Total CD19⁺ B cells B4 (× 10³)	Total CD34⁺CD19⁺ B-cell precursors GFP (× 10³)	Total CD34⁺CD19⁺ B-cell precursors B4 (× 10³)
3 GFs	3 wk	55.5 ± 39	94 ± 45	0.09 ± 0.09	3 ± 0.5
		(9.5 ± 6.4%)^*	(31.3 ± 15.1%)^*
	6 wk	1.6 ± 0.2	31.2 ± 3.5^†	0	0.5 ± 0.5^†
		(0.7 ± 0.4%)^*	(11.3 ± 2.2%)^*
6 GFs	3 wk	8.6 ± 7.2	67.8 ± 13.4^†	0.2 ± 0.06	4 ± 0.09^†
		(0.2 ± 0.1%)^*	(1.4 ± 0.2%)^*
	6 wk	1.6 ± 0.7	4.3 ± 0.1^†	0	0
		(0.1 ± 0.03%)^*	(0.3 ± 0.01%)^*

HOXB4-GFP and GFP-transduced CB cells were seeded on MS-5 murine fibroblasts and cultured with 3 (SCF, IL-2, and IL-15) or 6 (SCF, IL-2, IL-15, IL-7, FL, and TPO) growth factors permissive for B lymphoid in addition to variable degrees of myeloid cell growth (6 GFs > 3 GFs for myeloid growth). Hematopoietic differentiation into the lymphoid lineage was quantified by CD19 expression or CD34/CD19 coexpression (n = 2).

GF indicates growth factor.

Proportion of CD19⁺ B cells expressed as percentage of all cells in the culture. Nonlymphoid cells were predominantly of myeloid phenotype as assessed by FACS using a range of lineage markers (data not shown).

†

Statistically significant P < .03.

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