Table 1. Primers used for... | American Society of Hematology

Table 1.

Primers used for oligonucleotide-directed mutagenesis

Variant plasmid	Oligonucleotide sequence (5′ → 3′)
γ379	T A T T C C A T G T A G A A A A C C A C
γ384	A C C A C T A T G T A G A T A A T C C C
γ385	C C A C T A T G A A GT A A A T C C C A T T C A A C
γ386	C A C T A T G A A G A T A T A G C C A T T C A A C A G A C
γ387	C T A T G A A G A T A A T C T G A T T C A A C A G A C T C
γ390	C C A T T C A A C T G A C T C A C A A T T G
γ395	C A C A A T T G G A T A A G G A C A G C A
γ401	C A A C A C C A C T A G G G G G G A G C
γ406	G G A G C C A A A T A G G C T G G A G A

Variant plasmid	Oligonucleotide sequence (5′ → 3′)
γ379	T A T T C C A T G T A G A A A A C C A C
γ384	A C C A C T A T G T A G A T A A T C C C
γ385	C C A C T A T G A A GT A A A T C C C A T T C A A C
γ386	C A C T A T G A A G A T A T A G C C A T T C A A C A G A C
γ387	C T A T G A A G A T A A T C T G A T T C A A C A G A C T C
γ390	C C A T T C A A C T G A C T C A C A A T T G
γ395	C A C A A T T G G A T A A G G A C A G C A
γ401	C A A C A C C A C T A G G G G G G A G C
γ406	G G A G C C A A A T A G G C T G G A G A

The altered bases are indicated in bold characters.