Table 1. The most frequent V H genes... | American Society of Hematology

Table 1.

The most frequent V_H genes used in unmutated and mutated B-CLL across 4 studies

Homology to germline V_H gene	Unmutated/mutated cases, by study^*
Homology to germline V_H gene	No. 1	No. 2	No. 3	No. 4
V_H1-69
Below 98%	21/0	8/2	5/1	9/1
Below 96%	NM	8/2	5/1	9/1
V_H3-07
Below 98%	0/4	0/5	1/8	0/5
Below 96%	NM	1/4	1/8	0/5
V_H3-21
Below 98%	2/13	0/1	0/0	1/1
Below 96%	12/3	0/1	0/0	1/1
V_H3-23
Below 98%	0/3	0/7	2/1	3/8
Below 96%	NM	1/6	2/1	4/7
V_H3-30
Below 98%	2/4	3/8	2/0	6/4
Below 96%	NM	4/4	2/0	6/4
Total number of patients	119	74	64	84
Overall frequency of cases, unmutated/mutated cases, %
Below 98%	58/42	35/65	48/52	45/55
Below 96%	NM	47/53	55/45	57/43
Template type on which analysis was performed	DNA	DNA	RNA	RNA or DNA
Forward primer(s)	V_H1 to V_H6	FR1/2^†	V_HL1 to V_HL6	V_H1 to V_H6
Reverse primers(s)	J_H	JH cons^†	C_H	J_H

Homology to germline V_H gene	Unmutated/mutated cases, by study^*
Homology to germline V_H gene	No. 1	No. 2	No. 3	No. 4
V_H1-69
Below 98%	21/0	8/2	5/1	9/1
Below 96%	NM	8/2	5/1	9/1
V_H3-07
Below 98%	0/4	0/5	1/8	0/5
Below 96%	NM	1/4	1/8	0/5
V_H3-21
Below 98%	2/13	0/1	0/0	1/1
Below 96%	12/3	0/1	0/0	1/1
V_H3-23
Below 98%	0/3	0/7	2/1	3/8
Below 96%	NM	1/6	2/1	4/7
V_H3-30
Below 98%	2/4	3/8	2/0	6/4
Below 96%	NM	4/4	2/0	6/4
Total number of patients	119	74	64	84
Overall frequency of cases, unmutated/mutated cases, %
Below 98%	58/42	35/65	48/52	45/55
Below 96%	NM	47/53	55/45	57/43
Template type on which analysis was performed	DNA	DNA	RNA	RNA or DNA
Forward primer(s)	V_H1 to V_H6	FR1/2^†	V_HL1 to V_HL6	V_H1 to V_H6
Reverse primers(s)	J_H	JH cons^†	C_H	J_H

NM indicates not mentioned.

Study 1 is Tobin et al1; study 2, AZ Sint-Jan, Brugge, unpublished data, 2002; study 3, Fais et al2; study 4, Hamblin et al.3

Aubin et al.6