Table 1. Polymerase chain reaction... | American Society of Hematology

Table 1.

Polymerase chain reaction primers used for backtracking genomic AML1-ETO translocations in 10 patients

Patient	PCR round	AML1 primers (5′-3′)	ETO primers (5′-3′)	Product size
1	1	CTGCCATTCCGCTGACTAAG	AGATTCTCACCTAAAAGTCTGACT	126
	2	CTGCCATTCCGCTGACTAAG	GCAGCGGGGCCTAGCTGAAT	102
2^*	1	GTCTTCACAAACCCACCGCAAGTCG	CCTCCACATCTAGAAAGCAGGTCTACAGCAC	165
	2	CCCGAGAACCTCGAAGTAAGTGCAT	CCTCCACATCTAGAAAGCAGGTCTACAGCAC	91
3	1	GGGAGCAGAAGTGAGATGGT	CTAAATACTGTACCCAAGAAGGG	541
	2	GGTTGAGGCAGAATGCAAAAGTTCAAG	GGCTGCTTTATCCAGCGAACCC	503
4	1	TACCGTACATTATGGCTATATTATCA	AATGCCGAATAATTTATTCACCACCACC	132
	2	GCCAGTGACATTTAGTTGGATGTGAAAT	CCCATCCTGTTCAAGGTTAACCC	69
5^*	1	AGGGCCTAATCTTCACTCAGCTAC	AACGCGCATTGATGAATGGTC	170
	2	CTTGGTTGAGATCATGTCATATGAG	AACGCGCATTGATGAATGGTC	125
6	1	GGAAGCTCACCAGATAGGCT	GTACTATACTTATGTTAGAAAGAAAACA	195
	2	AGCTACCTCTGGGCTCCAGTG	CACAGTGGGAAACCCAACCTTTCAT	141
7	1	ATAATAAAATTCCAAGCAAGTACTTTCA	AAAGCTTAAAGCTACACATGGTC	447
	2	AATTTAGGACCTTTTTCAACTCTGCTTAAG	CCACAATTCTCCCCATTCAGTTTTCAG	320
8^*	1	GAAGCGGCAGTAACCCAGGTG	CCAGGTTCAATGGACTCTTCCC	249
	2	GTGCAGCTCTGAGTGAGCTGG	GGCTATTTTCTTCTCAGCAGTCCAG	136
9	1	CTTAGCTTAACGATATGAGAAGAAAT	CTAGCCTTCACCTATGACTTATG	238
	2	GTTACGTAGCACTGGACAATATATAAGCA	CTCAGGTCACTTTGATTTTTGCATCTCC	147
10	1	AACCACTGAAATTTGAGTCTTGAG	AGGATATGGAGCCAAGATCTCT	381
	2	CTGGCCCTGCTAGCTCAGTCAA	CCAAATTGTATAATAAAAATAGACAATCAATAACATG	215

Patient	PCR round	AML1 primers (5′-3′)	ETO primers (5′-3′)	Product size
1	1	CTGCCATTCCGCTGACTAAG	AGATTCTCACCTAAAAGTCTGACT	126
	2	CTGCCATTCCGCTGACTAAG	GCAGCGGGGCCTAGCTGAAT	102
2^*	1	GTCTTCACAAACCCACCGCAAGTCG	CCTCCACATCTAGAAAGCAGGTCTACAGCAC	165
	2	CCCGAGAACCTCGAAGTAAGTGCAT	CCTCCACATCTAGAAAGCAGGTCTACAGCAC	91
3	1	GGGAGCAGAAGTGAGATGGT	CTAAATACTGTACCCAAGAAGGG	541
	2	GGTTGAGGCAGAATGCAAAAGTTCAAG	GGCTGCTTTATCCAGCGAACCC	503
4	1	TACCGTACATTATGGCTATATTATCA	AATGCCGAATAATTTATTCACCACCACC	132
	2	GCCAGTGACATTTAGTTGGATGTGAAAT	CCCATCCTGTTCAAGGTTAACCC	69
5^*	1	AGGGCCTAATCTTCACTCAGCTAC	AACGCGCATTGATGAATGGTC	170
	2	CTTGGTTGAGATCATGTCATATGAG	AACGCGCATTGATGAATGGTC	125
6	1	GGAAGCTCACCAGATAGGCT	GTACTATACTTATGTTAGAAAGAAAACA	195
	2	AGCTACCTCTGGGCTCCAGTG	CACAGTGGGAAACCCAACCTTTCAT	141
7	1	ATAATAAAATTCCAAGCAAGTACTTTCA	AAAGCTTAAAGCTACACATGGTC	447
	2	AATTTAGGACCTTTTTCAACTCTGCTTAAG	CCACAATTCTCCCCATTCAGTTTTCAG	320
8^*	1	GAAGCGGCAGTAACCCAGGTG	CCAGGTTCAATGGACTCTTCCC	249
	2	GTGCAGCTCTGAGTGAGCTGG	GGCTATTTTCTTCTCAGCAGTCCAG	136
9	1	CTTAGCTTAACGATATGAGAAGAAAT	CTAGCCTTCACCTATGACTTATG	238
	2	GTTACGTAGCACTGGACAATATATAAGCA	CTCAGGTCACTTTGATTTTTGCATCTCC	147
10	1	AACCACTGAAATTTGAGTCTTGAG	AGGATATGGAGCCAAGATCTCT	381
	2	CTGGCCCTGCTAGCTCAGTCAA	CCAAATTGTATAATAAAAATAGACAATCAATAACATG	215

PCR indicates polymerase chain reaction.

Patients born and diagnosed in the United Kingdom. The remainder were born and diagnosed in California. Breakpoints for most patients were previously reported in Xiao et al.13 Patient 1 listed in this Table corresponds to patient no. 13 in Xiao, et al. Patient 2 is previous patient no. 1; patient 3, previous no. 15; patient 4, previous no. 9; patient 5, previous no. 6; patient 6, previous no. 19; patient 7, previous no. 14. Patients no. 8 and 10 are the same in both studies.

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