Table 1. Chelation of extracellular Ca 2... | American Society of Hematology

Table 1.

Chelation of extracellular Ca²⁺ variably affects the [Ca²⁺] rise induced by nucleotides in immature DCs

	ATP	UTP	ADP	BzATP	αβ-meATP	Control
Medium	1.43 ± 0.04	1.34 ± 0.02	1.34 ± 0.06	1.37 ± 0.05	1.39 ± 0.03	0.89 ± 0.04
EGTA	1.28 ± 0.03^*	1.31 ± 0.04	1.32 ± 0.03	0.96 ± 0.06^*	0.98 ± 0.07^*	0.85 ± 0.06

	ATP	UTP	ADP	BzATP	αβ-meATP	Control
Medium	1.43 ± 0.04	1.34 ± 0.02	1.34 ± 0.06	1.37 ± 0.05	1.39 ± 0.03	0.89 ± 0.04
EGTA	1.28 ± 0.03^*	1.31 ± 0.04	1.32 ± 0.03	0.96 ± 0.06^*	0.98 ± 0.07^*	0.85 ± 0.06

Immature DCs were loaded with Fura-2/am and stimulated with the indicated nucleotides (all 10⁻⁴ M) in the absence or in the presence of 4 mM EGTA. Results are expressed as the ratios between stimulated and unstimulated DCs after 10 seconds (± SEM, n = 5).

P < .002 versus untreated DCs.

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