Actin polymerization and chemotaxis of immature DCs to nucleotides are inhibited by pretreatment with PT
| . | Control . | ATP . | UTP . | ADP . |
|---|---|---|---|---|
| Relative f-actin* | ||||
| Medium | 1.00 ± 0.00 | 2.00 ± 0.17 | 1.81 ± 0.09 | 1.77 ± 0.10 |
| PT | 1.00 ± 0.00 | 1.16 ± 0.14‡ | 1.09 ± 0.08‡ | 1.08 ± 0.10‡ |
| Chemotaxis index† | ||||
| Medium | 1.00 ± 0.00 | 1.63 ± 0.15 | 1.57 ± 0.11 | 1.52 ± 0.08 |
| PT | 1.00 ± 0.00 | 1.04 ± 0.12‡ | 1.03 ± 0.10‡ | 1.06 ± 0.04‡ |
| . | Control . | ATP . | UTP . | ADP . |
|---|---|---|---|---|
| Relative f-actin* | ||||
| Medium | 1.00 ± 0.00 | 2.00 ± 0.17 | 1.81 ± 0.09 | 1.77 ± 0.10 |
| PT | 1.00 ± 0.00 | 1.16 ± 0.14‡ | 1.09 ± 0.08‡ | 1.08 ± 0.10‡ |
| Chemotaxis index† | ||||
| Medium | 1.00 ± 0.00 | 1.63 ± 0.15 | 1.57 ± 0.11 | 1.52 ± 0.08 |
| PT | 1.00 ± 0.00 | 1.04 ± 0.12‡ | 1.03 ± 0.10‡ | 1.06 ± 0.04‡ |
Immature DCs were stimulated or not with the indicated nucleotides (all at 10−4 M) and then evaluated for actin polymerization and chemotactic response. DCs were incubated or not with 4 μg/mL PT for 2 hours prior to the addition of nucleotides.
The content of filamentous actin was analyzed by flow cytometry following staining with NBD-phallacidin. Results are expressed as mean relative fluorescence intensity (± SEM; n, 3) at 25 seconds.
DC migration was evaluated in Transwell chambers with 5-μm pore size membranes. Results are expressed as mean (± SEM, n, 3) chemotactic index.
P < .002 vs untreated DCs.