Mitoxantrone accumulation studies in MCF-7 and MCF-7 MR cell lines
| . | Mitoxantrone accumulation, MFI . | FTC shift, MFI . | PSC833 shift, MFI . | P-gp activity . | MK-571 shift, MFI . | MRP activity . |
|---|---|---|---|---|---|---|
| MCF-7 | 211 ± 15 | 86 ± 14 | 52 ± 6 | 1.2 ± 0.3 | 89 ± 14 | 3.3 ± 0.3 |
| MCF-7 MR | 93* ± 36 | 254* ± 54 | 23 ± 9 | 1.1 ± 0.2 | 54 ± 16 | 4.6 ± 0.9 |
| MCF-7 Dox40 | 109* ± 41 | 22* ± 22 | 242† ± 93 | 20.3† ± 2.0 | 36 ± 39 | 7.6 ± 0.9 |
| . | Mitoxantrone accumulation, MFI . | FTC shift, MFI . | PSC833 shift, MFI . | P-gp activity . | MK-571 shift, MFI . | MRP activity . |
|---|---|---|---|---|---|---|
| MCF-7 | 211 ± 15 | 86 ± 14 | 52 ± 6 | 1.2 ± 0.3 | 89 ± 14 | 3.3 ± 0.3 |
| MCF-7 MR | 93* ± 36 | 254* ± 54 | 23 ± 9 | 1.1 ± 0.2 | 54 ± 16 | 4.6 ± 0.9 |
| MCF-7 Dox40 | 109* ± 41 | 22* ± 22 | 242† ± 93 | 20.3† ± 2.0 | 36 ± 39 | 7.6 ± 0.9 |
The results represent mean ± SD of at least 3 experiments. The concentrations used are as follows: mitoxantrone, 10 μM; FTC, 10 μM; PSC833, 2 μg/mL; and MK-571, 20 μM. P-gp activity is expressed as PSC833 efflux-blocking factor of Rh123. MRP activity is expressed as MK-571 efflux-blocking factor of CF.
P < .05 compared with MCF-7.
P < .05 compared with MCF-7 and MCF-7 MR.