Table 1. Ex vivo analysis of plasma... | American Society of Hematology

Table 1.

Ex vivo analysis of plasma samples after administration of 100 and 300 μg/kg 82D6A3 to baboons

Time	Platelet count (10³/mm³)	Bleeding time (min)	VWF-Ag levels (μg/mL)	82D6A3 levels (μg/mL)	VWF occupancy (%)	Collagen binding (%)
100 μg/kg (n = 3)
0	286 ± 54	2.7 ± 0.4	10.2 ± 1.7	0	2.3 ± 1.3	101 ± 7
30 min	292 ± 65	2.7 ± 0.4	10.2 ± 2.5	0.4 ± 0.07^†	80 ± 10.8^†	64 ± 7^*
60 min	289 ± 49	3.5 ± 2.1	8.9 ± 1.4	0.4 ± 0.1^*	80 ± 2.4^‡	69 ± 9
300 μg/kg (n = 3)
0	286 ± 54	2.7 ± 0.4	10.2 ± 1.7	0	2.3 ± 1.3	101 ± 7
30 min	265 ± 41	4.6 ± 0.6	8.8 ± 1.4	2.9 ± 0.3^†	102 ± 10.4^†	4 ± 1^†
60 min	287 ± 53	7.3 ± 2.5	9.1 ± 2.4	2.8 ± 0.3^†	99 ± 10.6^†	4 ± 1^†
150 min	309 ± 83	6.4 ± 3.1	9.7 ± 2.7	2.6 ± 0.1^‡	101 ± 7.6^†	4 ± 1^†
300 min	282 ± 7	3.15 ± 1.2	8.8 ± 0.1	2.0 ± 0.5	94 ± 0.9^†	4 ± 1^†
24 h	312 ± 46	3.25 ± 0.3	12.8 ± 1.3	0.7 ± 0.2^*	74 ± 31	91 ± 18
48 h	306 ± 79	3	13.2 ± 0.8	0.2 ± 0.01^*	63 ± 7.8^*	93 ± 0

Time	Platelet count (10³/mm³)	Bleeding time (min)	VWF-Ag levels (μg/mL)	82D6A3 levels (μg/mL)	VWF occupancy (%)	Collagen binding (%)
100 μg/kg (n = 3)
0	286 ± 54	2.7 ± 0.4	10.2 ± 1.7	0	2.3 ± 1.3	101 ± 7
30 min	292 ± 65	2.7 ± 0.4	10.2 ± 2.5	0.4 ± 0.07^†	80 ± 10.8^†	64 ± 7^*
60 min	289 ± 49	3.5 ± 2.1	8.9 ± 1.4	0.4 ± 0.1^*	80 ± 2.4^‡	69 ± 9
300 μg/kg (n = 3)
0	286 ± 54	2.7 ± 0.4	10.2 ± 1.7	0	2.3 ± 1.3	101 ± 7
30 min	265 ± 41	4.6 ± 0.6	8.8 ± 1.4	2.9 ± 0.3^†	102 ± 10.4^†	4 ± 1^†
60 min	287 ± 53	7.3 ± 2.5	9.1 ± 2.4	2.8 ± 0.3^†	99 ± 10.6^†	4 ± 1^†
150 min	309 ± 83	6.4 ± 3.1	9.7 ± 2.7	2.6 ± 0.1^‡	101 ± 7.6^†	4 ± 1^†
300 min	282 ± 7	3.15 ± 1.2	8.8 ± 0.1	2.0 ± 0.5	94 ± 0.9^†	4 ± 1^†
24 h	312 ± 46	3.25 ± 0.3	12.8 ± 1.3	0.7 ± 0.2^*	74 ± 31	91 ± 18
48 h	306 ± 79	3	13.2 ± 0.8	0.2 ± 0.01^*	63 ± 7.8^*	93 ± 0

Data are mean data ± SD. At each time point, the plasma samples were measured 3 times in 3 different ELISAs for the 3 animal experiments. For statistical analysis, the mean data were used. Studentt test (paired) was used for statistical evaluation.

P < .05.

†

P < .01.

‡

P < .001.

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