Ex vivo analysis of plasma samples after administration of 600 μg/kg 82D6A3 to baboons
| . | Platelet count (103/mm3) . | Bleeding time (min) . | VWF-Ag levels (μg/mL) . | 82D6A3 levels (μg/mL) . | VWF occupancy (%) . | Collagen binding (%) . |
|---|---|---|---|---|---|---|
| 0 min | 335 | 1.8 | 14 ± 1.7 | 0 | 6.9 ± 0.1 | 100 ± 0 |
| 30 min | 320 | 3.5 | 11.5 ± 0.9 | 4.5 ± 0.5 | 96 ± 1 | 4 ± 0.2 |
| 60 min | 313 | 5.5 | 10.8 ± 0.1 | 4.8 ± 0.7 | 96 ± 0.2 | 3.5 ± 0.2 |
| 150 min | 356 | 5 | 11.9 ± 1.8 | 3.8 ± 0.5 | 97 ± 4 | 3.5 ± 0.2 |
| 300 min | 334 | 3 | 10.5 | 3.8 ± 0.6 | 97 | 4 |
| 24 h | 347 | ND | 22.9 | 1.4 ± 0.01 | 88 | 45 |
| . | Platelet count (103/mm3) . | Bleeding time (min) . | VWF-Ag levels (μg/mL) . | 82D6A3 levels (μg/mL) . | VWF occupancy (%) . | Collagen binding (%) . |
|---|---|---|---|---|---|---|
| 0 min | 335 | 1.8 | 14 ± 1.7 | 0 | 6.9 ± 0.1 | 100 ± 0 |
| 30 min | 320 | 3.5 | 11.5 ± 0.9 | 4.5 ± 0.5 | 96 ± 1 | 4 ± 0.2 |
| 60 min | 313 | 5.5 | 10.8 ± 0.1 | 4.8 ± 0.7 | 96 ± 0.2 | 3.5 ± 0.2 |
| 150 min | 356 | 5 | 11.9 ± 1.8 | 3.8 ± 0.5 | 97 ± 4 | 3.5 ± 0.2 |
| 300 min | 334 | 3 | 10.5 | 3.8 ± 0.6 | 97 | 4 |
| 24 h | 347 | ND | 22.9 | 1.4 ± 0.01 | 88 | 45 |
Data are mean data ± SD. At each time point, the plasma samples were measured 3 times in 3 different ELISAs for the 2 animal experiments. ND indicates not determined.