Primer sequences used for PCR amplification of individual exons of CASP10
| Name . | Exon . | Sense primer sequence (5′-3′)* . | Antisense primer sequence (5′-3′) . | Product length (bp) . |
|---|---|---|---|---|
| E2A | 2 | gggccatatgtcctcactctc | AAACTTGAGGTTCTCCACATCTTG | 184 |
| E2B | 2 | CTTTCGTGAGAAGCTTCTGATTAT | TTCTGCCGTATGATATAGAGGAGT | 210 |
| E2C | 2 | TGCTGAGTGAGGAAGACCCTTTCT | ctcccatctccaccacagacc | 169 |
| E3 | 3 | cttacaagtgtaaggctttattt | cattgattaagacagtgctcaca | 191 |
| E4 | 4 | tgagtggataatcaataggcaagt | ctccaagttagcaatcacaagc | 217 |
| E5 | 5 | actgcaacctccgcctcctg | cattgaccagcacaccactgaacc | 224 |
| E6 | 6 | gtccttccctgcatcaagtc | ccctaccataccgatctaagttgt | 173 |
| E7 | 7 | tggggaagatatttggagtctgag | gcccctaaagaaaccgtcctt | 213 |
| E8 | 8 | Aaggattcctactaagtggctcta | gcttttgataaactgttccaga | 177 |
| E9A | 9 | tgtgcccggccttgtttcag | GGGCTGGATTGCACTTCTGCTTCT | 214 |
| E9B | 9 | CGAAAGTGGAAATGGAGATGGT | CAGGCCTGGATGAAAAAGAGTTTA | 220 |
| E9C | 9 | GGGAGATCATGTCTCACTTCACA | CCACATGCCGAAAGGATACA | 234 |
| E9D | 9 | CTACTTGGTCTGGCCACTGT | taccaaaggtgttgaatgagagta | 189 |
| E10 | 10 | aaattttgttttcttctttgttgc | caatgattcgtttgaggtctaag | 222 |
| E11 | 11 | ttccccttttatttctctttgtgc | gtcaatctcaggcgatgtgg | 237 |
| Name . | Exon . | Sense primer sequence (5′-3′)* . | Antisense primer sequence (5′-3′) . | Product length (bp) . |
|---|---|---|---|---|
| E2A | 2 | gggccatatgtcctcactctc | AAACTTGAGGTTCTCCACATCTTG | 184 |
| E2B | 2 | CTTTCGTGAGAAGCTTCTGATTAT | TTCTGCCGTATGATATAGAGGAGT | 210 |
| E2C | 2 | TGCTGAGTGAGGAAGACCCTTTCT | ctcccatctccaccacagacc | 169 |
| E3 | 3 | cttacaagtgtaaggctttattt | cattgattaagacagtgctcaca | 191 |
| E4 | 4 | tgagtggataatcaataggcaagt | ctccaagttagcaatcacaagc | 217 |
| E5 | 5 | actgcaacctccgcctcctg | cattgaccagcacaccactgaacc | 224 |
| E6 | 6 | gtccttccctgcatcaagtc | ccctaccataccgatctaagttgt | 173 |
| E7 | 7 | tggggaagatatttggagtctgag | gcccctaaagaaaccgtcctt | 213 |
| E8 | 8 | Aaggattcctactaagtggctcta | gcttttgataaactgttccaga | 177 |
| E9A | 9 | tgtgcccggccttgtttcag | GGGCTGGATTGCACTTCTGCTTCT | 214 |
| E9B | 9 | CGAAAGTGGAAATGGAGATGGT | CAGGCCTGGATGAAAAAGAGTTTA | 220 |
| E9C | 9 | GGGAGATCATGTCTCACTTCACA | CCACATGCCGAAAGGATACA | 234 |
| E9D | 9 | CTACTTGGTCTGGCCACTGT | taccaaaggtgttgaatgagagta | 189 |
| E10 | 10 | aaattttgttttcttctttgttgc | caatgattcgtttgaggtctaag | 222 |
| E11 | 11 | ttccccttttatttctctttgtgc | gtcaatctcaggcgatgtgg | 237 |
Upper case letters correspond to exons and lower case letters correspond to introns.