Procoagulant activity of peripheral blood monocytes
. | Monocyte procoagulant activity (au) . | |
---|---|---|
15-min incubation . | 4-h incubation . | |
Control medium | 19.5 ± 17.5 | 21.9 ± 17 |
EMP | 65.6 ± 32.2* | 173 ± 84† |
EMP-free supernatant | 20 ± 14 | 23 ± 9 |
LPS (1 μg/mL) | 28 ± 12 | 821 ± 97† |
. | Monocyte procoagulant activity (au) . | |
---|---|---|
15-min incubation . | 4-h incubation . | |
Control medium | 19.5 ± 17.5 | 21.9 ± 17 |
EMP | 65.6 ± 32.2* | 173 ± 84† |
EMP-free supernatant | 20 ± 14 | 23 ± 9 |
LPS (1 μg/mL) | 28 ± 12 | 821 ± 97† |
Peripheral blood monocytes from healthy volunteers were incubated with EMP, control medium, LPS (1 μg/mL), or supernatant free of EMP for 15 minutes and 4 hours and then were prepared for assessment of procoagulant activity using clotting assay. Procoagulant activity was determined by reference to a thromboplastin standard curve and was expressed as arbitrary units. Data are mean ± SD (n = 4).
P < .05 compared with control medium;
P < .01 compared with control medium.