Cytogenetic/FISH investigations in the 21 patients with CD4+, CD56+ DC2 acute leukemia
| UPN . | Sex/age . | Source of cells/culture (h) . | % blasts BM . | FISH/probes . | Karyotype according to R-bands and as modified after 24-color karyotyping . |
|---|---|---|---|---|---|
| 1* | M/76 | BM/24 | 63 | — | 46,XY[40] |
| 2* | M/8 | BM/24 | 70 | M-FISH | 44,X,-Y,del(1)(q21),der(3),del(5)(q3?3q3?5),add(6)(q2?1),-13[7]]/46,XY[8] |
| Ish wcp M-FISH 44,X,-Y,der(1)t(Y;1)(q12;q?21),der(3)t(1;3)(p11;q?),del(5)(q3?3q3?5), der(6)(6pter->6q22::1q?::8q?),der(12)t(1;12)(?;p11),r(13)[3] | |||||
| 3 | F/86 | BM/24 | 87 | M-FISH | 46,XX,del(5)(q13q33)[2]/42,idem,add(1)(q41),add(2)(q37),-9,-12,-13,-15,-20,-21,add (21)(q22),+2mar[20]/46XX[3] |
| ish wcp M-FISH der(1)t(1;8)(q4?3;?),dup(2)(?q35q37),-9,der(12)(?),-13,-15,der(20;21) (?p11;?q22),der(21)(21pter->21qter::12::21q11->21qter),-21[3] | |||||
| 4 | F/81 | BM/24 & 48 | 44 | — | 84-87,XX,del(X)(q24qter)x2[11],add(2)(q3?)[11],der(2)(?)[11],+del(4)(q23qter)[9], +del(5)(q14qter)[11],+del(5)(q14q23)[11],add(6)(q?),-9[11],-11[6],del(12)(p12){3],-13[11],-13[9],-14[11],-15x2[11],-17[11],-17[5],-18[3],+r(?)[11],+mar1[11},+mar2[4], +mar3[3][cp11]/46,XX[4] |
| 6 | M/68 | BM/24 | 40 | M-FISH | 46,XY[21]. ish wcp M-FISH[30] |
| 7 | M/75 | BM/24 | 74 | M-FISH | 45,XY,-5,add(7)(p22)[3]/46,XY[10]. |
| Ish wcp M-FISH del(5)(q3?1q3?5),der(7)(12qter->12q1?1::7q10->7qter),-12[3] | |||||
| 8 | M/79 | LN/17 | 74 | M-FISH | 45,XY,-5,add(6)(q2?2),del(11)(q14q23),-13,-15,+2mar[4]/46,XY[2]. |
| ish wcp M FISH 45,XY,+5,der(5)t(5;11)(p10;?)x2,add(6)(q2?2),del(11)(q14q23),-13, -15[4] | |||||
| 9 | F/6 | BM/24 & 48 | 90 | — | 44,XX,del(3)(p21),-5,-12,-13,add(17)(p11),-18,-19,+3mar[27]/46,XX[2] |
| 10 | M/55 | BM/24 | 80 | 13q14 | 46,XY[25].Ish 13q14(D13S25)x2)[100%] |
| 11* | M/79 | BM/24 | 0 | Wcp5/6 | 48,XY,+6,add(6)(q10)x2,add(9)(p24),der(12)t(1;12)(q22;p13),+21[2]/46,XY[13]. |
| ish 5(wcp5x2),add(6)(wcp6+) | |||||
| 12 | M/14 | BM/17 | 96 | SKY wcp3, 19 Cen 7/8, Tel3p,3q,6p,6q | 43,XY,add(3p2?3),add(6)(q?21),-7,-9,add(12)(p11),-13,-19,+mar[7]/86,idemx2[3].ish SKY 43,XY,t(3;6)(p25;q2?3),der(7)t(7;19)(7qter->7p21::19q10->19qter),-9, der(12)t(5;12)(?;p11),-13,-19[10] |
| 13* | M/74 | PB/24 | 0 | M-FISH | 46,XY,del(2)(p2?1),add(8)(q2?4),del(13)(q1?3q2?1)[31]/47,idem,+mar[10]. ish wcp M-FISH +Y(wcpY+)[10] |
| 14 | F/79 | BM/24 | 74 | TEL/AML1 | 46,XX,del(12)(p12p13)[9]/46,XX[2]. |
| 5q31, 13q14 | ish/nuc ish12p13(TEL/ETV6x1),21q22(AML1x2)[78%], 5q31(248FSx2), 13q14(D13S319x2)[100%] | ||||
| 15 | M/67 | BM/24 | 60 | TEL/AML1 | 46,XY[20]. nuc ish 12p13(TEL/ETV6x2),21q22(AML1x2) |
| 16 | F/75 | BM/48 | 90 | — | 45,XX,del(5)(q13q34),?inv(11)(p11q21),der(15)(15qter->15q10::?::18q10->18qter), -18[15]/44,idem,-22,dmin[2]/88,idemx2,del(6)(q16)[2]/46,XX[1] |
| 17 | M/60 | BM/24 | 100 | — | Failure |
| 18 | M/82 | PB/24 | 92 | M-FISH | 42,X,-Y,-2,del(5)(p13),del(5)(q13q33),del(6)(q24),-11,-13,add(13)(p11),-14,add(14) (p11),-15,add(18)(q21),add(19)(q13),-21,-22,+4mar[18]/46,XY[3] |
| ish wcp M-FISH 42,X,-Y,der(2)(6qter-6q1?1::2p10->2p?::5?),del(5)(p13),der(5) (5pter->5q21::13q?),der(6)(6pter->6q1?1::2q?14->2qter),t(6;18)(q2?2;q22), der(11),-13,der(13)t(13;21)(q10;q10),-14,der(14)t(Y;14)(q11;p11),r(15),der(19) (19pter->19q13::3p21->3pter),-21,i(22)(q10)[3] | |||||
| 19 | M/56 | BM/72 | 52 | — | 46,XY[21] |
| 20 | M/77 | BM/96 | 21 | — | 46,XY[40] |
| 21 | M/70 | BM/24 | 48 | — | 44,XY,-9,-13[8]/88,idemx2[18]/46,XY[5] |
| 24 | M/74 | PB/24 | 95 | M-FISH wcp 6, 8, 15, 16 | 49,XY,+add(6)(q21),-8,+2mar,+r[6]/49,idem,t(15;16)(?q21;?q21)[6]/49,idem,t(3;5) (?q21;?q31)[5] |
| ish wcp M-FISH 49,XY,+6,t(6;8)(p21;q24),+r(12),+20/49,idem,inv(15)(q1?4q2?3), t(16;16)(q?;q?)/49,idem,t(3;5)(q?21;q?31)[13] |
| UPN . | Sex/age . | Source of cells/culture (h) . | % blasts BM . | FISH/probes . | Karyotype according to R-bands and as modified after 24-color karyotyping . |
|---|---|---|---|---|---|
| 1* | M/76 | BM/24 | 63 | — | 46,XY[40] |
| 2* | M/8 | BM/24 | 70 | M-FISH | 44,X,-Y,del(1)(q21),der(3),del(5)(q3?3q3?5),add(6)(q2?1),-13[7]]/46,XY[8] |
| Ish wcp M-FISH 44,X,-Y,der(1)t(Y;1)(q12;q?21),der(3)t(1;3)(p11;q?),del(5)(q3?3q3?5), der(6)(6pter->6q22::1q?::8q?),der(12)t(1;12)(?;p11),r(13)[3] | |||||
| 3 | F/86 | BM/24 | 87 | M-FISH | 46,XX,del(5)(q13q33)[2]/42,idem,add(1)(q41),add(2)(q37),-9,-12,-13,-15,-20,-21,add (21)(q22),+2mar[20]/46XX[3] |
| ish wcp M-FISH der(1)t(1;8)(q4?3;?),dup(2)(?q35q37),-9,der(12)(?),-13,-15,der(20;21) (?p11;?q22),der(21)(21pter->21qter::12::21q11->21qter),-21[3] | |||||
| 4 | F/81 | BM/24 & 48 | 44 | — | 84-87,XX,del(X)(q24qter)x2[11],add(2)(q3?)[11],der(2)(?)[11],+del(4)(q23qter)[9], +del(5)(q14qter)[11],+del(5)(q14q23)[11],add(6)(q?),-9[11],-11[6],del(12)(p12){3],-13[11],-13[9],-14[11],-15x2[11],-17[11],-17[5],-18[3],+r(?)[11],+mar1[11},+mar2[4], +mar3[3][cp11]/46,XX[4] |
| 6 | M/68 | BM/24 | 40 | M-FISH | 46,XY[21]. ish wcp M-FISH[30] |
| 7 | M/75 | BM/24 | 74 | M-FISH | 45,XY,-5,add(7)(p22)[3]/46,XY[10]. |
| Ish wcp M-FISH del(5)(q3?1q3?5),der(7)(12qter->12q1?1::7q10->7qter),-12[3] | |||||
| 8 | M/79 | LN/17 | 74 | M-FISH | 45,XY,-5,add(6)(q2?2),del(11)(q14q23),-13,-15,+2mar[4]/46,XY[2]. |
| ish wcp M FISH 45,XY,+5,der(5)t(5;11)(p10;?)x2,add(6)(q2?2),del(11)(q14q23),-13, -15[4] | |||||
| 9 | F/6 | BM/24 & 48 | 90 | — | 44,XX,del(3)(p21),-5,-12,-13,add(17)(p11),-18,-19,+3mar[27]/46,XX[2] |
| 10 | M/55 | BM/24 | 80 | 13q14 | 46,XY[25].Ish 13q14(D13S25)x2)[100%] |
| 11* | M/79 | BM/24 | 0 | Wcp5/6 | 48,XY,+6,add(6)(q10)x2,add(9)(p24),der(12)t(1;12)(q22;p13),+21[2]/46,XY[13]. |
| ish 5(wcp5x2),add(6)(wcp6+) | |||||
| 12 | M/14 | BM/17 | 96 | SKY wcp3, 19 Cen 7/8, Tel3p,3q,6p,6q | 43,XY,add(3p2?3),add(6)(q?21),-7,-9,add(12)(p11),-13,-19,+mar[7]/86,idemx2[3].ish SKY 43,XY,t(3;6)(p25;q2?3),der(7)t(7;19)(7qter->7p21::19q10->19qter),-9, der(12)t(5;12)(?;p11),-13,-19[10] |
| 13* | M/74 | PB/24 | 0 | M-FISH | 46,XY,del(2)(p2?1),add(8)(q2?4),del(13)(q1?3q2?1)[31]/47,idem,+mar[10]. ish wcp M-FISH +Y(wcpY+)[10] |
| 14 | F/79 | BM/24 | 74 | TEL/AML1 | 46,XX,del(12)(p12p13)[9]/46,XX[2]. |
| 5q31, 13q14 | ish/nuc ish12p13(TEL/ETV6x1),21q22(AML1x2)[78%], 5q31(248FSx2), 13q14(D13S319x2)[100%] | ||||
| 15 | M/67 | BM/24 | 60 | TEL/AML1 | 46,XY[20]. nuc ish 12p13(TEL/ETV6x2),21q22(AML1x2) |
| 16 | F/75 | BM/48 | 90 | — | 45,XX,del(5)(q13q34),?inv(11)(p11q21),der(15)(15qter->15q10::?::18q10->18qter), -18[15]/44,idem,-22,dmin[2]/88,idemx2,del(6)(q16)[2]/46,XX[1] |
| 17 | M/60 | BM/24 | 100 | — | Failure |
| 18 | M/82 | PB/24 | 92 | M-FISH | 42,X,-Y,-2,del(5)(p13),del(5)(q13q33),del(6)(q24),-11,-13,add(13)(p11),-14,add(14) (p11),-15,add(18)(q21),add(19)(q13),-21,-22,+4mar[18]/46,XY[3] |
| ish wcp M-FISH 42,X,-Y,der(2)(6qter-6q1?1::2p10->2p?::5?),del(5)(p13),der(5) (5pter->5q21::13q?),der(6)(6pter->6q1?1::2q?14->2qter),t(6;18)(q2?2;q22), der(11),-13,der(13)t(13;21)(q10;q10),-14,der(14)t(Y;14)(q11;p11),r(15),der(19) (19pter->19q13::3p21->3pter),-21,i(22)(q10)[3] | |||||
| 19 | M/56 | BM/72 | 52 | — | 46,XY[21] |
| 20 | M/77 | BM/96 | 21 | — | 46,XY[40] |
| 21 | M/70 | BM/24 | 48 | — | 44,XY,-9,-13[8]/88,idemx2[18]/46,XY[5] |
| 24 | M/74 | PB/24 | 95 | M-FISH wcp 6, 8, 15, 16 | 49,XY,+add(6)(q21),-8,+2mar,+r[6]/49,idem,t(15;16)(?q21;?q21)[6]/49,idem,t(3;5) (?q21;?q31)[5] |
| ish wcp M-FISH 49,XY,+6,t(6;8)(p21;q24),+r(12),+20/49,idem,inv(15)(q1?4q2?3), t(16;16)(q?;q?)/49,idem,t(3;5)(q?21;q?31)[13] |
BM indicates bone marrow; PB, peripheral blood; LN, lymph node; Cen, centromeric probe; Tel, telomeric probe.
Cytogenetic data obtained in the course of the secondary medullary dissemination.