Mean fluorescence intensity values and percentage positive for mAb staining on BCD-treated cells
| mAb specificity . | mAb clone . | MFI (% positive) . | |
|---|---|---|---|
| Control . | BCD . | ||
| Nonspecific | mIgG2b | 2.92 (2.10) | 3.18 (4.87) |
| CD4 | RPA-T4 | 403.9 (100) | 410.9 (99.9) |
| CD45 | HI30 | 1425 (100) | 1563 (100) |
| CCR5, N-terminal | CTC5 | 214.8 (100) | 106.3 (99.1) |
| CCR5, N-terminal | 45502 | 10.03 (69.0) | 7.55 (58.7) |
| CCR5, MD | 45523 | 56.8 (97.8) | 43.6 (97.8) |
| CCR5, MD | 45549 | 8.54 (77.2) | 5.16 (32.8) |
| CCR5, ECL2 | 45531 | 393.6 (99.5) | 56.1 (98.3) |
| mAb specificity . | mAb clone . | MFI (% positive) . | |
|---|---|---|---|
| Control . | BCD . | ||
| Nonspecific | mIgG2b | 2.92 (2.10) | 3.18 (4.87) |
| CD4 | RPA-T4 | 403.9 (100) | 410.9 (99.9) |
| CD45 | HI30 | 1425 (100) | 1563 (100) |
| CCR5, N-terminal | CTC5 | 214.8 (100) | 106.3 (99.1) |
| CCR5, N-terminal | 45502 | 10.03 (69.0) | 7.55 (58.7) |
| CCR5, MD | 45523 | 56.8 (97.8) | 43.6 (97.8) |
| CCR5, MD | 45549 | 8.54 (77.2) | 5.16 (32.8) |
| CCR5, ECL2 | 45531 | 393.6 (99.5) | 56.1 (98.3) |
MFI, mean fluorescence intensity; mAb, monoclonal antibody; BCD, hydroxypropyl-β-cyclodextrin; MD, multidomain; ECL, extracellular loop.
Cells were treated with 20 mM BCD or untreated in phosphate-buffered saline for 1 hour at 37°C. The cells were then washed and stained for flow cytometry as described in “Materials and methods.” The data presented here are from histograms in Figure 4.