Mean fluorescence intensity values and percentage positive for mAb staining on BCD-treated cells fixed with paraformaldehyde
| mAb specificity . | . | MFI (% positive) . | |
|---|---|---|---|
| mAb clone . | Control . | BCD . | |
| Nonspecific | mIgG2b | 3.54 (2.0) | 27.4 (30.9) |
| CD45 | HI30 | 1116 (100) | 1249 (100) |
| CCR5, N-terminal | CTC5 | 192 (98.7) | 766.9 (99.9) |
| CCR5, N-terminal | 45502 | 5.71 (15.7) | 250.6 (96.0) |
| CCR5, MD | 45523 | 57.69 (94.8) | 166 (99.7) |
| CCR5, MD | 45549 | 5.84 (22.1) | 241 (96.1) |
| CCR5, ECL2 | 45531 | 123.6 (97.9) | 132.8 (99.6) |
| mAb specificity . | . | MFI (% positive) . | |
|---|---|---|---|
| mAb clone . | Control . | BCD . | |
| Nonspecific | mIgG2b | 3.54 (2.0) | 27.4 (30.9) |
| CD45 | HI30 | 1116 (100) | 1249 (100) |
| CCR5, N-terminal | CTC5 | 192 (98.7) | 766.9 (99.9) |
| CCR5, N-terminal | 45502 | 5.71 (15.7) | 250.6 (96.0) |
| CCR5, MD | 45523 | 57.69 (94.8) | 166 (99.7) |
| CCR5, MD | 45549 | 5.84 (22.1) | 241 (96.1) |
| CCR5, ECL2 | 45531 | 123.6 (97.9) | 132.8 (99.6) |
MFI, mean fluorescence intensity; mAb, monoclonal antibodies; BCD, beta-cyclodextrin; MD, ; ECL, extracellular loop.
Cells were treated with BCD as described in “Materials and methods” and then fixed with 2% paraformaldehyde prior to staining with mAbs. The data presented here are from histograms in Figure 5.