Characteristics of specific genetic markers analyzed by real-time quantitative polymerase chain reaction
| Marker name . | Location . | Informativity, % . | Position . | 5′ Primer 3′ . | TaqMan (FAM-TAMRA) probe . |
|---|---|---|---|---|---|
| S 01a | 17q | 23.6 | F | GGTACCGGGTCTCCACATGA | CTGGGCCAGAATCTTGGTCCTCACA |
| R* | GGGAAAGTCACTCACCCAAGG | ||||
| S 01b | F | GTACCGGGTCTCCACCAGG | |||
| S 02 | Y | 36.4 | F | GCTTCTCTGGTTGGAGTCACG | CTGCACCACCAAATCATCCCCGTG |
| R | GCTTGCTGGCGGACCCT | ||||
| S 03 | 6q | 16.4 | F | CTTTTGCTTTCTGTTTCTTAAGGGC | CATACGTGCACAGGGTCCCCGAGT |
| R | TCAATCTTTGGGCAGGTTGAA | ||||
| S 04a | 9 | 23.6 | F* | CTGGTGCCCACAGTTACGCT | TCCTGGCAGTGTGGTCCCTTCAGAA |
| R | AAGGATGCGTGACTGCTATGG | ||||
| S 04b | 12.7 | R | AGGATGCGTGACTGCTCCTC | ||
| S 05a | 20 | 1.8 | F | AAAGTAGACACGGCCAGACTTAGG | CCCTGGACACTGAAAACAGGCAATCCT |
| R* | CATCCCCACATACGGAAAAGA | ||||
| S 05b | 27.3 | F | AGTTAAAGTAGACACGGCCTCCC | ||
| S 06 | 1p | 31.0 | F | CAGTCACCCCGTGAAGTCCT | CCCATCCATCTTCCCTACCAGACCAGG |
| R | TTTCCCCCATCTGCCTATTG | ||||
| S 07a | X | 31.0 | F | TGGTATTGGCTTTAAAATACTGGG | TCCTCACTTCTCCACCCCTAGTTAAACAG |
| R | TGTACCCAAAACTCAGCTGCA | ||||
| S 07b | 21.8 | F | GGTATTGGCTTTAAAATACTCAACC | ||
| R | CAGCTGCAACAGTTATCAACGTT | ||||
| S 08a | 1q | 16.4 | F | CTGGATGCCTCACTGATCCA | CTCCCAACCCCCATTTCTGCCTG |
| R* | TGGGAAGGATGCATATGATCTG | ||||
| S 08b | 20.2 | F | GCTGGATGCCTCACTGATGTT | ||
| S 09a | 17q | 7.3 | F* | GGGCACCCGTGTGAGTTTT | TGGAGGATTTCTCCCCTGCTTCAGACAG |
| R | TCAGCTTGTCTGCTTTCTGGAA | ||||
| S 09b | 14.5 | R | CAGCTTGTCTGCTTTCTGCTG | ||
| S 10a | 18 | 25.5 | F | GCCACAAGAGACTCAG | CAGTGTCCCACTCAAGTACTCCTTTGGA |
| R | TGGCTTCCTTGAGGTGGAAT | ||||
| S 10b | 20.2 | F | TTAGAGCCACAAGAGACAACCAG | ||
| S 11a | 11 | 25.5 | F | TAGGATTCAACCCTGGAAGC | CAAGGCTTCCTCAATTCTCCACCCTTCC |
| R* | CCAGCATGCACCTGACTAACA | ||||
| S 11b | 7.3 | F | CCCTGGATCGCCGTGAA | ||
| GAPDH | F | GGACTGAGGCTCCCACCTTT | CATCCAAGACTGGCTCCTCCCTGC | ||
| R | GCATGGACTGTGGTCTGCAA |
| Marker name . | Location . | Informativity, % . | Position . | 5′ Primer 3′ . | TaqMan (FAM-TAMRA) probe . |
|---|---|---|---|---|---|
| S 01a | 17q | 23.6 | F | GGTACCGGGTCTCCACATGA | CTGGGCCAGAATCTTGGTCCTCACA |
| R* | GGGAAAGTCACTCACCCAAGG | ||||
| S 01b | F | GTACCGGGTCTCCACCAGG | |||
| S 02 | Y | 36.4 | F | GCTTCTCTGGTTGGAGTCACG | CTGCACCACCAAATCATCCCCGTG |
| R | GCTTGCTGGCGGACCCT | ||||
| S 03 | 6q | 16.4 | F | CTTTTGCTTTCTGTTTCTTAAGGGC | CATACGTGCACAGGGTCCCCGAGT |
| R | TCAATCTTTGGGCAGGTTGAA | ||||
| S 04a | 9 | 23.6 | F* | CTGGTGCCCACAGTTACGCT | TCCTGGCAGTGTGGTCCCTTCAGAA |
| R | AAGGATGCGTGACTGCTATGG | ||||
| S 04b | 12.7 | R | AGGATGCGTGACTGCTCCTC | ||
| S 05a | 20 | 1.8 | F | AAAGTAGACACGGCCAGACTTAGG | CCCTGGACACTGAAAACAGGCAATCCT |
| R* | CATCCCCACATACGGAAAAGA | ||||
| S 05b | 27.3 | F | AGTTAAAGTAGACACGGCCTCCC | ||
| S 06 | 1p | 31.0 | F | CAGTCACCCCGTGAAGTCCT | CCCATCCATCTTCCCTACCAGACCAGG |
| R | TTTCCCCCATCTGCCTATTG | ||||
| S 07a | X | 31.0 | F | TGGTATTGGCTTTAAAATACTGGG | TCCTCACTTCTCCACCCCTAGTTAAACAG |
| R | TGTACCCAAAACTCAGCTGCA | ||||
| S 07b | 21.8 | F | GGTATTGGCTTTAAAATACTCAACC | ||
| R | CAGCTGCAACAGTTATCAACGTT | ||||
| S 08a | 1q | 16.4 | F | CTGGATGCCTCACTGATCCA | CTCCCAACCCCCATTTCTGCCTG |
| R* | TGGGAAGGATGCATATGATCTG | ||||
| S 08b | 20.2 | F | GCTGGATGCCTCACTGATGTT | ||
| S 09a | 17q | 7.3 | F* | GGGCACCCGTGTGAGTTTT | TGGAGGATTTCTCCCCTGCTTCAGACAG |
| R | TCAGCTTGTCTGCTTTCTGGAA | ||||
| S 09b | 14.5 | R | CAGCTTGTCTGCTTTCTGCTG | ||
| S 10a | 18 | 25.5 | F | GCCACAAGAGACTCAG | CAGTGTCCCACTCAAGTACTCCTTTGGA |
| R | TGGCTTCCTTGAGGTGGAAT | ||||
| S 10b | 20.2 | F | TTAGAGCCACAAGAGACAACCAG | ||
| S 11a | 11 | 25.5 | F | TAGGATTCAACCCTGGAAGC | CAAGGCTTCCTCAATTCTCCACCCTTCC |
| R* | CCAGCATGCACCTGACTAACA | ||||
| S 11b | 7.3 | F | CCCTGGATCGCCGTGAA | ||
| GAPDH | F | GGACTGAGGCTCCCACCTTT | CATCCAAGACTGGCTCCTCCCTGC | ||
| R | GCATGGACTGTGGTCTGCAA |
Eleven biallelic genetic systems were used (S 01 to S 11). For 3 genetic systems (S 02, S 03, S 06), only one allele was found to be informative. Chromosome location, percentage of informativity evaluated on 63 donor/recipient pairs, and sequence of probes and primers are indicated. In the majority of cases (all except the S 07 system), one primer was common for both alleles. GAPDH was the constant genetic system used as active reference.
S 01, S 03, and S 04 markers were selected from references 31, 32, and 33, respectively.
FAM indicates 6-carboxyfluorescein; TAMRA, 6-carboxy-tetramethyl-rhodamine; F, forward primer; R, reverse primer; GAPDH, glyceraldehyde phosphate dehydrogenase.
Common primer.