Comparison of short tandem repeat polymerase chain reaction and real-time polymerase chain reaction results
| Effective recipient cells, % . | Measured recipient genotype, % . | ||||
|---|---|---|---|---|---|
| STR-PCR . | Real-time PCR . | ||||
| Tho1 . | D21S11 . | S 08b . | S 09a . | S 11a . | |
| 80 | 78 | 72 | 58 | 65 | 65 |
| 40 | 40 | 31 | 29 | 35 | 30 |
| 20 | 25 | 13 | 14 | 16 | 15 |
| 10 | 12 | 5 | 7 | 6 | 8 |
| 5 | 7 | 1.3 | 4 | 4 | 5.5 |
| 2.5 | 6 | nd | 1.20 | 2.50 | 1.40 |
| 1.25 | nd | nd | 0.80 | 0.99 | 0.73 |
| 0.62 | nd | nd | 0.42 | 0.40 | 0.63 |
| 0.31 | nd | nd | 0.16 | 0.21 | 0.22 |
| 0.15 | nd | nd | 0.08 | 0.11 | 0.14 |
| 0.07 | nd | nd | 0.06 | 0.07 | 0.07 |
| Effective recipient cells, % . | Measured recipient genotype, % . | ||||
|---|---|---|---|---|---|
| STR-PCR . | Real-time PCR . | ||||
| Tho1 . | D21S11 . | S 08b . | S 09a . | S 11a . | |
| 80 | 78 | 72 | 58 | 65 | 65 |
| 40 | 40 | 31 | 29 | 35 | 30 |
| 20 | 25 | 13 | 14 | 16 | 15 |
| 10 | 12 | 5 | 7 | 6 | 8 |
| 5 | 7 | 1.3 | 4 | 4 | 5.5 |
| 2.5 | 6 | nd | 1.20 | 2.50 | 1.40 |
| 1.25 | nd | nd | 0.80 | 0.99 | 0.73 |
| 0.62 | nd | nd | 0.42 | 0.40 | 0.63 |
| 0.31 | nd | nd | 0.16 | 0.21 | 0.22 |
| 0.15 | nd | nd | 0.08 | 0.11 | 0.14 |
| 0.07 | nd | nd | 0.06 | 0.07 | 0.07 |
Effective percentage of recipient cells in donor cells for the 11 artificial cell mixtures and recipient genotype percentages measured with 2 informative markers by short tandem repeat–polymerase chain reaction assay and 3 recipient-specific markers by real-time polymerase chain reaction assay are indicated.
STR indicates short tandem repeat; PCR, polymerase chain reaction; nd, recipient genotype not detected.