Primers used to construct GPIbβ/IX chimeras
| Chimera name . | 1st-round beta primer . | 1st-round GPIX primer . | ||
|---|---|---|---|---|
| Name . | Sequence . | Name . | Sequence . | |
| GPIX22/15GPIbβ | sBeta140 | 5′-atggggctgtgggtggactgcGGGCGCCGCGGGCTGACTTGG | aIX 61 | 5′-ccaagtcagcccgcggcgcccGCAGTCCACCCACAGCCCCAT |
| GPIX36/32GPIbβ | sBeta 191 | 5′-tgcctgccctgccggcccgcACAACCGAGCTGGTGCTGACC | aIX 103 | 5′-tcagcsccagctcggttgtGCGGGCCGGCAGGGCAGGCAG |
| GPIX53/49GPIbβ | sBeta 242 | 5′-ttcagtccgtgcccccgggaCTGCTGGACGCGCTGCCCGCG | aIX 157 | 5′-agcgcgggcagcgcgtccagcagTCCCGGGGGCACGGACTG |
| Chimera name . | 1st-round beta primer . | 1st-round GPIX primer . | ||
|---|---|---|---|---|
| Name . | Sequence . | Name . | Sequence . | |
| GPIX22/15GPIbβ | sBeta140 | 5′-atggggctgtgggtggactgcGGGCGCCGCGGGCTGACTTGG | aIX 61 | 5′-ccaagtcagcccgcggcgcccGCAGTCCACCCACAGCCCCAT |
| GPIX36/32GPIbβ | sBeta 191 | 5′-tgcctgccctgccggcccgcACAACCGAGCTGGTGCTGACC | aIX 103 | 5′-tcagcsccagctcggttgtGCGGGCCGGCAGGGCAGGCAG |
| GPIX53/49GPIbβ | sBeta 242 | 5′-ttcagtccgtgcccccgggaCTGCTGGACGCGCTGCCCGCG | aIX 157 | 5′-agcgcgggcagcgcgtccagcagTCCCGGGGGCACGGACTG |
The overhangs are in bold.
GP indicates glycoprotein.