Sequences of primers and TaqMan probes
| Primer designation . | Sequence (5′-3′) . |
|---|---|
| ATM forward | TGGATCCAGCTATTTGGTTTGA |
| ATM reverse | CCAAGTATGTAACCAACAATAGAAGAAGTAG |
| BAX forward | CCTTTTCTACTTTGCCAGCAAAC |
| BAX reverse | GAGGCCGTCCCAACCAC |
| BCL2 forward | GGCTGGGATGCCTTTGTG |
| BCL2 reverse | GCCAGGAGAAATCAAACAGAGG |
| CCND1 forward | CTGGAGGTCTGCGAGGAACA |
| CCND1 TaqMan probe | AGGTCTTCCCGCTGGCCATGAACTAC |
| CCND1 reverse | TTTTTCACGGGCTCCAGC |
| CCND3 forward | GCCCTCTGTGCTACAGATTATACCT |
| CCND3 reverse | TGCTGCCCGTGGCG |
| CDK2 forward | GCTAGCAGACTTTGGACTAGCCAG |
| CDK2 reverse | AGCTCGGTACCACAGGGTCA |
| CDK4 forward | ATGTTGTCCGGCTGATGGA |
| CDK4 reverse | CACCAGGGTTACCTTGATCTCC |
| CDKN1A forward | CGCTAATGGCGGGCTG |
| CDKN1A reverse | CGGTGACAAAGTCGAAGTTCC |
| CDKN1B forward | CTGCAACCGACGATTCTTCTACT |
| CDKN1B reverse | GGGCGTCTGCTCCACAGA |
| E2F1 forward | AGATGGTTATGGTGATCAAAGCC |
| E2F1 reverse | ATCTGAAAGTTCTCCGAAGAGTCC |
| ETV5 forward | GCGGCCTGTGATTGACAGA |
| ETV5 reverse | GGAACTTGTGCTTCAGCTAACCA |
| LMNB1 forward | GATTGCCCAGTTGGAAGCCT |
| LMNB1 reverse | TGGTCTCGTTAATCTCCTCTTCATACA |
| MYC forward | TGCCAGGACCCGCTTCT |
| MYC TaqMan probe | CAGCTGCTTAGACGCTGGATTTTTTTCG |
| MYC reverse | GAGGCTGCTGGTTTTCCACTA |
| PGK1 forward | AAGTGAAGCTCGGAAAGCTTCTAT |
| PGK1 reverse | AGGGAAAAGATGCTTCTGGG |
| RB1 forward | CTTGCATGGCTCTCAGATTCAC |
| RB1 TaqMan probe | ATTAAACAATCAAAGGACCGAGAAGGACCAACTG |
| RB1 reverse | AGAGGACAAGCAGATTCAAGGTG |
| SELL forward | TCACGTCGTCTTCTGTATACTGTGG |
| SELL reverse | TTGCAGCTAGCATTTCAGTGATG |
| TFDP2 forward | TCAGGCAAATGCTCTCTGGAG |
| TFDP2 reverse | ACCAAGAAGGTCCTGTGGAGATAT |
| TNFSF10 forward | GAGCTGAAGCAGATGCAGGAC |
| TNFSF10 reverse | TGACGGAGTTGCCACTTGACT |
| TP53 forward | CCCTTCAGATCCGTGGGC |
| TP53 TaqMan probe | TCTGTCCCCCTTGCCGTCCCA |
| TP53 reverse | TGAGTTCCAAGGCCTCATTCA |
| Primer designation . | Sequence (5′-3′) . |
|---|---|
| ATM forward | TGGATCCAGCTATTTGGTTTGA |
| ATM reverse | CCAAGTATGTAACCAACAATAGAAGAAGTAG |
| BAX forward | CCTTTTCTACTTTGCCAGCAAAC |
| BAX reverse | GAGGCCGTCCCAACCAC |
| BCL2 forward | GGCTGGGATGCCTTTGTG |
| BCL2 reverse | GCCAGGAGAAATCAAACAGAGG |
| CCND1 forward | CTGGAGGTCTGCGAGGAACA |
| CCND1 TaqMan probe | AGGTCTTCCCGCTGGCCATGAACTAC |
| CCND1 reverse | TTTTTCACGGGCTCCAGC |
| CCND3 forward | GCCCTCTGTGCTACAGATTATACCT |
| CCND3 reverse | TGCTGCCCGTGGCG |
| CDK2 forward | GCTAGCAGACTTTGGACTAGCCAG |
| CDK2 reverse | AGCTCGGTACCACAGGGTCA |
| CDK4 forward | ATGTTGTCCGGCTGATGGA |
| CDK4 reverse | CACCAGGGTTACCTTGATCTCC |
| CDKN1A forward | CGCTAATGGCGGGCTG |
| CDKN1A reverse | CGGTGACAAAGTCGAAGTTCC |
| CDKN1B forward | CTGCAACCGACGATTCTTCTACT |
| CDKN1B reverse | GGGCGTCTGCTCCACAGA |
| E2F1 forward | AGATGGTTATGGTGATCAAAGCC |
| E2F1 reverse | ATCTGAAAGTTCTCCGAAGAGTCC |
| ETV5 forward | GCGGCCTGTGATTGACAGA |
| ETV5 reverse | GGAACTTGTGCTTCAGCTAACCA |
| LMNB1 forward | GATTGCCCAGTTGGAAGCCT |
| LMNB1 reverse | TGGTCTCGTTAATCTCCTCTTCATACA |
| MYC forward | TGCCAGGACCCGCTTCT |
| MYC TaqMan probe | CAGCTGCTTAGACGCTGGATTTTTTTCG |
| MYC reverse | GAGGCTGCTGGTTTTCCACTA |
| PGK1 forward | AAGTGAAGCTCGGAAAGCTTCTAT |
| PGK1 reverse | AGGGAAAAGATGCTTCTGGG |
| RB1 forward | CTTGCATGGCTCTCAGATTCAC |
| RB1 TaqMan probe | ATTAAACAATCAAAGGACCGAGAAGGACCAACTG |
| RB1 reverse | AGAGGACAAGCAGATTCAAGGTG |
| SELL forward | TCACGTCGTCTTCTGTATACTGTGG |
| SELL reverse | TTGCAGCTAGCATTTCAGTGATG |
| TFDP2 forward | TCAGGCAAATGCTCTCTGGAG |
| TFDP2 reverse | ACCAAGAAGGTCCTGTGGAGATAT |
| TNFSF10 forward | GAGCTGAAGCAGATGCAGGAC |
| TNFSF10 reverse | TGACGGAGTTGCCACTTGACT |
| TP53 forward | CCCTTCAGATCCGTGGGC |
| TP53 TaqMan probe | TCTGTCCCCCTTGCCGTCCCA |
| TP53 reverse | TGAGTTCCAAGGCCTCATTCA |