Cells tolerized to alloantigen inhibit cytokine production by naive alloreactive T cells but not naive DO11 CD4+cells exposed to optimal ovalbumin concentration
| Culture day . | Group . | IL-2 . | IFN-γ . | IL-4 . | IL-10 . | IL-13 . |
|---|---|---|---|---|---|---|
| 3 | Naive anti-B6 | 419 | 34 | 11 | 0 | 0 |
| 3 | Naive + tolerized anti-B6 | 3 | 7 | 4 | 0 | 0 |
| 3 | DO11, OVA + BALB/c APCs | 1703 | 1163 | 59 | 40 | 62 |
| 3 | DO11, OVA, tolerized CD4+ + BALB/c APCs | 1732 | 1218 | 41 | 28 | 88 |
| 5 | Naive anti-B6 | 943 | 142 | 14 | 5 | 1 |
| 5 | Naive + tolerized anti-B6 | 2 | 8 | 9 | 8 | 0 |
| 5 | DO11, OVA + BALB/c APCs | 1749 | 1112 | 122 | 2449 | 1033 |
| 5 | DO11, OVA, tolerized CD4+ + BALB/c APCs | 1662 | 1506 | 65 | 2092 | 992 |
| Culture day . | Group . | IL-2 . | IFN-γ . | IL-4 . | IL-10 . | IL-13 . |
|---|---|---|---|---|---|---|
| 3 | Naive anti-B6 | 419 | 34 | 11 | 0 | 0 |
| 3 | Naive + tolerized anti-B6 | 3 | 7 | 4 | 0 | 0 |
| 3 | DO11, OVA + BALB/c APCs | 1703 | 1163 | 59 | 40 | 62 |
| 3 | DO11, OVA, tolerized CD4+ + BALB/c APCs | 1732 | 1218 | 41 | 28 | 88 |
| 5 | Naive anti-B6 | 943 | 142 | 14 | 5 | 1 |
| 5 | Naive + tolerized anti-B6 | 2 | 8 | 9 | 8 | 0 |
| 5 | DO11, OVA + BALB/c APCs | 1749 | 1112 | 122 | 2449 | 1033 |
| 5 | DO11, OVA, tolerized CD4+ + BALB/c APCs | 1662 | 1506 | 65 | 2092 | 992 |
Primary lymphocyte reaction (MLR) cultures were established either with naive BALB/c CD4+ T cells and irradiated, T cell-depleted B6 splenic stimulators or with DO11 CD4+ T cells, ovalbumin peptide at 5.0 μg/mL, and irradiated BALB/c splenic antigen-presenting cells. BALB/c CD4+ cells that were tolerized to B6 alloantigen were added to some wells. Supernatants were harvested on days 3 and 5 of culture. Final concentration of naive BALB/c CD4+ cells was 0.5 × 106 cells per milliliter. Final concentration of DO11 CD4+ cells was 0.15 × 106 cells per milliliter. Tolerized cells were added at a ratio of 1 tolerized cell to 3 naive BALB/c CD4+ cells and 1 tolerized cell to 1 DO11 CD4+ cell. Values are in pg per milliliter.
IL indicates interleukin; IFN, interferon; OVA, ovalbumin; APCs, antigen-presenting cells.