Reversible binding properties of the Arg67His mutant thrombin toward synthetic and natural substrates/ligands
| . | WT . | Arg67His . | . |
|---|---|---|---|
| Binding to TM and platelet activation | Kd(nM) | Kd(nM) | ΔΔG* (Kcal/mol) |
| TM binding | 2.1 ± 0.2 | 65.3 ± 7 | 2.1 |
| EC50 (nM) | EC50(nM) | ||
| Platelet activation | 2.1 ± 0.2 | 20.4 ± 1.3 | 1.4 |
| . | WT . | Arg67His . | . |
|---|---|---|---|
| Binding to TM and platelet activation | Kd(nM) | Kd(nM) | ΔΔG* (Kcal/mol) |
| TM binding | 2.1 ± 0.2 | 65.3 ± 7 | 2.1 |
| EC50 (nM) | EC50(nM) | ||
| Platelet activation | 2.1 ± 0.2 | 20.4 ± 1.3 | 1.4 |
For TM binding and platelet activation the ΔΔG value was calculated using the Kd and EC50value, respectively.