Kinetic parameters pertaining to inhibition of WT and Arg67His thrombin forms by AT and HCII in the absence and presence of GAGs*
| AT inhibition . | WT . | Arg67His . |
|---|---|---|
| kon (M−1s−1) | kon (M−1s−1) | |
| No GAG | 1.1 ± 0.2 × 104 | 1.0 ± 0.1 × 104 |
| With GAG | 8.0 ± 0.1 × 107 | 5.2 ± 0.5 × 107 |
| HCII inhibition | ||
| kon(M−1 s−1) | kon(M−1 s−1) | |
| No GAG | 1.0 ± 0.2 × 103 | 0.35 ± 0.05 × 103 |
| With GAG | 5.1 ± 0.4 × 107 | 1.2 ± 0.2 × 105 |
| AT inhibition . | WT . | Arg67His . |
|---|---|---|
| kon (M−1s−1) | kon (M−1s−1) | |
| No GAG | 1.1 ± 0.2 × 104 | 1.0 ± 0.1 × 104 |
| With GAG | 8.0 ± 0.1 × 107 | 5.2 ± 0.5 × 107 |
| HCII inhibition | ||
| kon(M−1 s−1) | kon(M−1 s−1) | |
| No GAG | 1.0 ± 0.2 × 103 | 0.35 ± 0.05 × 103 |
| With GAG | 5.1 ± 0.4 × 107 | 1.2 ± 0.2 × 105 |
GAG is represented by high-molecular-weight heparin used at 70 nM in AT experiments, and by 25 μM dermatan sulphate in inhibition experiments with HCII.