Differentiation of amplified peripheral blood B cells into CD20−/CD38++ cells
| Healthy donors . | CD20−/ CD38++(%) . | Annexin-V (%) . | Amplification factor from day 4 to 6 . | Overall amplification factor . |
|---|---|---|---|---|
| 1 | 57 | 18 | 2.6 | 6.8 |
| 2 | 50 | 30 | 4.2 | 9.5 |
| 3 | 41 | 24 | 3 | 6.4 |
| 4 | 60 | 22 | 3.7 | 15.6 |
| 5 | 41 | 30 | 2.6 | 7.7 |
| 6 | 42 | 32 | 3 | 9 |
| 7 | 51 | 26 | 4 | 9.4 |
| 8 | 46 | 29 | 2.8 | 7.6 |
| 9 | 56 | 22 | 3.5 | 8 |
| 10 | 51 | 23 | 2.5 | 11 |
| Mean ± SD | 50 ± 7 | 25.6 ± 4.5 | 3.2 ± 0.6 | 9.1 ± 2.7 |
| Healthy donors . | CD20−/ CD38++(%) . | Annexin-V (%) . | Amplification factor from day 4 to 6 . | Overall amplification factor . |
|---|---|---|---|---|
| 1 | 57 | 18 | 2.6 | 6.8 |
| 2 | 50 | 30 | 4.2 | 9.5 |
| 3 | 41 | 24 | 3 | 6.4 |
| 4 | 60 | 22 | 3.7 | 15.6 |
| 5 | 41 | 30 | 2.6 | 7.7 |
| 6 | 42 | 32 | 3 | 9 |
| 7 | 51 | 26 | 4 | 9.4 |
| 8 | 46 | 29 | 2.8 | 7.6 |
| 9 | 56 | 22 | 3.5 | 8 |
| 10 | 51 | 23 | 2.5 | 11 |
| Mean ± SD | 50 ± 7 | 25.6 ± 4.5 | 3.2 ± 0.6 | 9.1 ± 2.7 |
Purified peripheral blood B cells from 10 healthy donors were cultured for 4 days with CD40 + IL-2 + IL-4 + IL-10 + IL-12. Amplified B cells were then harvested, washed, and cultured for 2 additional days with IL-2 + IL-10 + IL-12 + IL-6. At day 6 of culture, the viability was evaluated by annexin V staining, and the percentage of CD20−/CD38++ plasma cells was determined by flow cytometry.