Correction of aberrant splicing by modified U7 snRNAs in EGFP mutant HeLa cells
| . | FI of EGFP-654 . | FI of EGFP-705U . |
|---|---|---|
| snRNA | ||
| U7.623 | 1068 ± 52 | 1741 ± 59 |
| U7.324 | 786 ± 38 | 1186 ± 43 |
| PTKU7.324 | 18 ± 11 | 19 ± 12 |
| PTKU7.324r | 194 ± 56 | 360 ± 58 |
| PTKU7.623r | 349 ± 58 | 576 ± 60 |
| Control | ||
| U7SmOPT | 3 ± 3 | 7 ± 4 |
| . | FI of EGFP-654 . | FI of EGFP-705U . |
|---|---|---|
| snRNA | ||
| U7.623 | 1068 ± 52 | 1741 ± 59 |
| U7.324 | 786 ± 38 | 1186 ± 43 |
| PTKU7.324 | 18 ± 11 | 19 ± 12 |
| PTKU7.324r | 194 ± 56 | 360 ± 58 |
| PTKU7.623r | 349 ± 58 | 576 ± 60 |
| Control | ||
| U7SmOPT | 3 ± 3 | 7 ± 4 |
HeLa EGFP-654 and EGFP-705U cells transiently transfected with 0.25 μg U7 snRNA plasmids (3 independent experiments) were analyzed in terms of a fluorescence index (FI; percentage of cells scoring above the background threshold × mean fluorescence intensity of that cell subpopulation). Control, U7.SmOPT lacks the antisense sequence.