Analysis of pan-reactivity of anti-mIECSA (mAb 2H5/D3) and anti–DBL-γ3CSA (mAb 1B11/A5)
| Strain . | L-IFA . | % inhibition of cytoadhesion . | ||||
|---|---|---|---|---|---|---|
| 2H5/D3 . | 1B11/A5 . | CSA (100 μg/mL) . | Chondroitinase ABC (1 U/mL) . | |||
| Before panning . | After panning . | Before panning . | After panning . | |||
| B358 | 2 | > 94 | 2 | > 94 | 96 | 90 |
| BXII | 5 | > 94 | 5 | > 94 | 95 | 92 |
| FCBR | 5 | > 94 | 4 | > 94 | 95 | 96 |
| SUK | 98 | > 94 | 95 | > 94 | 90 | 91 |
| H | 2 | > 94 | 3 | > 94 | 92 | 93 |
| IBR | 97 | > 94 | 95 | > 94 | 90 | 95 |
| FCR3 | 0.3 | > 94 | 0.3 | > 94 | 91 | 96 |
| Strain . | L-IFA . | % inhibition of cytoadhesion . | ||||
|---|---|---|---|---|---|---|
| 2H5/D3 . | 1B11/A5 . | CSA (100 μg/mL) . | Chondroitinase ABC (1 U/mL) . | |||
| Before panning . | After panning . | Before panning . | After panning . | |||
| B358 | 2 | > 94 | 2 | > 94 | 96 | 90 |
| BXII | 5 | > 94 | 5 | > 94 | 95 | 92 |
| FCBR | 5 | > 94 | 4 | > 94 | 95 | 96 |
| SUK | 98 | > 94 | 95 | > 94 | 90 | 91 |
| H | 2 | > 94 | 3 | > 94 | 92 | 93 |
| IBR | 97 | > 94 | 95 | > 94 | 90 | 95 |
| FCR3 | 0.3 | > 94 | 0.3 | > 94 | 91 | 96 |
L-IFA with mIEs of 7 laboratory strains from different geographic regions. The results obtained with both mAbs are presented as the percentages of positive surface-stained mIEs in the initial population (before panning) and in the CSA-selected subpopulation (after panning on Scl7 Saimiri brain microvascular endothelial cells). The specificity of the panned cytoadhesion phenotype was confirmed by a cytoadhesion inhibition assay in the presence of soluble CSA and after chondroitinase ABC treatment of the endothelial cells. The observed inhibition of cytoadhesion was expressed as percentage inhibition with respect to the control.