Freshly isolated rat peritoneal and alveolar macrophages kill mM-CSF-transfected T9-C2 cells but not T9 or T9-H1 (sM-CSF)-transfected cells
| Macrophage:T9 ratio . | Specific release, %, ± SD . | ||
|---|---|---|---|
| T9 parental cells . | sM-CSF T9-H1 clone . | mM-CSF T9-C2 . | |
| Peritoneal macrophages | |||
| 10:1 | 10 ± 0 | Not tested | 32 ± 0* |
| 5:1 | 6 ± 0 | Not tested | 22 ± 1* |
| 2.5:1 | 5 ± 0 | Not tested | 18 ± 0* |
| Alveolar macrophages | |||
| 10:1 | 8 ± 1 | 2 ± 1 | 23 ± 5* |
| 5:1 | 5 ± 0 | 1 ± 2 | 21 ± 6* |
| 2.5:1 | 4 ± 2 | 0 ± 1 | 16 ± 0* |
| Macrophage:T9 ratio . | Specific release, %, ± SD . | ||
|---|---|---|---|
| T9 parental cells . | sM-CSF T9-H1 clone . | mM-CSF T9-C2 . | |
| Peritoneal macrophages | |||
| 10:1 | 10 ± 0 | Not tested | 32 ± 0* |
| 5:1 | 6 ± 0 | Not tested | 22 ± 1* |
| 2.5:1 | 5 ± 0 | Not tested | 18 ± 0* |
| Alveolar macrophages | |||
| 10:1 | 8 ± 1 | 2 ± 1 | 23 ± 5* |
| 5:1 | 5 ± 0 | 1 ± 2 | 21 ± 6* |
| 2.5:1 | 4 ± 2 | 0 ± 1 | 16 ± 0* |
Freshly isolated rat macrophages were added to the T9, T9-H1, and T9-C2 target cells and the assay was harvested after 24 hours.
These values were significantly different (P< .05) from the results of the T9 tumor cell killing.