Sensitivity of pyrosequencing at low levels of chimerism
| Donor/ recipient, % . | Donor cells detected ± SD, % . | Presence of donor SNP allele in replicate assays . |
|---|---|---|
| 50/50 | 50.4 ± 1.5 | 5/5 |
| 5/95 | 4.6 ± 3.3 | 5/5 |
| 2/98 | 0.6 ± 2.3 | 3/5 |
| 1/99 | 0 ± 2.6 | 2/5 |
| 0.5/99.5 | 0 ± 2.2 | 1/5 |
| 0/100 | 0 ± 1.5 | 0/5 |
| Donor/ recipient, % . | Donor cells detected ± SD, % . | Presence of donor SNP allele in replicate assays . |
|---|---|---|
| 50/50 | 50.4 ± 1.5 | 5/5 |
| 5/95 | 4.6 ± 3.3 | 5/5 |
| 2/98 | 0.6 ± 2.3 | 3/5 |
| 1/99 | 0 ± 2.6 | 2/5 |
| 0.5/99.5 | 0 ± 2.2 | 1/5 |
| 0/100 | 0 ± 1.5 | 0/5 |