Life span extension of CD4+ T-cell clones by hTERT transduction
CD4+ T-cell clone . | Subpopulation . | Replicative age,* PD . | Life span,† PD . | No. of experiments . |
|---|---|---|---|---|
| MoT-72 | Th1/Th0 | 30 | 42 | 3‡ |
| MoT-72 | Th1/Th0 | 34 | 42 | 2‡ |
| MoT-81 | Th1/Th0 | 31 | 43 | 2‡ |
| MoT-89 | Th1/Th0 | 28 | 38 | 1 |
| CBXL-2.12 | Th1 | 36 | NE | 1 |
| NAD.E178 | Th1 | 36 | NE | 1 |
| PEN J6 | Th2 | 41 | 56 | 1 |
| PEN J50 | Th2 | 42 | 55 | 1 |
| NP-12 | Th2 | 46 | 47 | 1 |
| NP-44 | Th2 | 34 | 36 | 1 |
| BOY JF157 | Th2 | 36 | NE | 2‡ |
| BOY JF161 | Tr | 36 | NE | 1 |
| BOY JF180 | Tr | 36 | NE | 1 |
CD4+ T-cell clone . | Subpopulation . | Replicative age,* PD . | Life span,† PD . | No. of experiments . |
|---|---|---|---|---|
| MoT-72 | Th1/Th0 | 30 | 42 | 3‡ |
| MoT-72 | Th1/Th0 | 34 | 42 | 2‡ |
| MoT-81 | Th1/Th0 | 31 | 43 | 2‡ |
| MoT-89 | Th1/Th0 | 28 | 38 | 1 |
| CBXL-2.12 | Th1 | 36 | NE | 1 |
| NAD.E178 | Th1 | 36 | NE | 1 |
| PEN J6 | Th2 | 41 | 56 | 1 |
| PEN J50 | Th2 | 42 | 55 | 1 |
| NP-12 | Th2 | 46 | 47 | 1 |
| NP-44 | Th2 | 34 | 36 | 1 |
| BOY JF157 | Th2 | 36 | NE | 2‡ |
| BOY JF161 | Tr | 36 | NE | 1 |
| BOY JF180 | Tr | 36 | NE | 1 |
NE indicates not exactly determined in PD.
Replicative age in population doublings (PD) of the T cells at the time of hTERT transduction. The T cells had undergone approximately 20 PDs during cloning to establish a clonal population.
Life span of the untransduced clones in PD.
Life span extension by hTERT was successful in all experiments.