hTERT-GFP transduction does not alter the cytokine production profile of CD4+ T-cell clones
T-cell clones . | Subpopulation . | IL-4 . | IL-5 . | IL-10 . | IFNγ . |
|---|---|---|---|---|---|
| NAD.E178 | Th1 | <0.05* | <0.05 | <0.05 | 9.7 |
| NAD.E178TERT | Th1 | <0.05 | <0.05 | <0.05 | 6.5 |
| CBXI-2. 12 | Th1 | 0.1 | <0.05 | 1.2 | 8.9 |
| CBXI-2. 12TERT | Th1 | <0.05 | <0.05 | 0.8 | 6.1 |
| BOY JF157 | Th2 | 1-11† | 76-105 | 0.3-9.7 | 0.05-0.1 |
| BOY JF157TERT | Th2 | 7-30 | 51-363 | 1-6.7 | 0.7-2.7 |
| BOY JF161 | Tr | 0.02 | 88 | 3.5 | 166 |
| BOY JF161TERT | Tr | 0.15 | 81 | 0.04 | 156 |
T-cell clones . | Subpopulation . | IL-4 . | IL-5 . | IL-10 . | IFNγ . |
|---|---|---|---|---|---|
| NAD.E178 | Th1 | <0.05* | <0.05 | <0.05 | 9.7 |
| NAD.E178TERT | Th1 | <0.05 | <0.05 | <0.05 | 6.5 |
| CBXI-2. 12 | Th1 | 0.1 | <0.05 | 1.2 | 8.9 |
| CBXI-2. 12TERT | Th1 | <0.05 | <0.05 | 0.8 | 6.1 |
| BOY JF157 | Th2 | 1-11† | 76-105 | 0.3-9.7 | 0.05-0.1 |
| BOY JF157TERT | Th2 | 7-30 | 51-363 | 1-6.7 | 0.7-2.7 |
| BOY JF161 | Tr | 0.02 | 88 | 3.5 | 166 |
| BOY JF161TERT | Tr | 0.15 | 81 | 0.04 | 156 |
CD4+ T-cell clones derived from the different subpopulations Th0, Th1, Th2, and Tr were transduced with hTERT-GFP, and the production of cytokines, compared with untransduced cells, upon stimulation with anti-CD3 and anti-CD28 antibodies was determined by ELISA.
Average cytokine production (ng per mL) as measured in triplicate by ELISA.
Ranges of values indicate the range of average cytokine production (ng per mL) detected in 3 different experiments during the culture.