G-CSF–induced phagocytosis of IgG-coated sheep RBCs by neutrophils treated or untreated with piceatannol
. | PI . | . | . | . | . | . | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
Exposure no. . | Untreated . | . | Piceatannol, 25 μg/mL . | . | Piceatannol, 50 μg/mL . | . | |||||
. | Control . | G-CSF . | Control . | G-CSF . | Control . | G-CSF . | |||||
1 | 62 | 86 | 40 | 68 | 20 | 49 | |||||
2 | 29 | 75 | 36 | 29 | 28 | 53 | |||||
3 | 25 | 122 | 30 | 35 | 9 | 4 | |||||
4 | 35 | 105 | 25 | 36 | 10 | 7 | |||||
Mean | 38 | 97 | 33 | 42 | 17 | 28 | |||||
SD | 17 | 21 | 7 | 18 | 9 | 26 | |||||
P | .03 | .29 | .29 |
. | PI . | . | . | . | . | . | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
Exposure no. . | Untreated . | . | Piceatannol, 25 μg/mL . | . | Piceatannol, 50 μg/mL . | . | |||||
. | Control . | G-CSF . | Control . | G-CSF . | Control . | G-CSF . | |||||
1 | 62 | 86 | 40 | 68 | 20 | 49 | |||||
2 | 29 | 75 | 36 | 29 | 28 | 53 | |||||
3 | 25 | 122 | 30 | 35 | 9 | 4 | |||||
4 | 35 | 105 | 25 | 36 | 10 | 7 | |||||
Mean | 38 | 97 | 33 | 42 | 17 | 28 | |||||
SD | 17 | 21 | 7 | 18 | 9 | 26 | |||||
P | .03 | .29 | .29 |
The PI for nonopsonized RBCs, based on the mean of 3 experiments, was 0 and 1 for cells not exposed to piceatannol, respectively not stimulated and stimulated with G-CSF (300 ng/mL); and 0 and 0 both for unstimulated and G-CSF—stimulated cells, both in the presence of 25 μg/mL or 50 μg/mL piceatannol.