Table 2. Sequence of primers used for... | American Society of Hematology

Table 2.

Sequence of primers used for RT-PCR

Probe	Sense	Antisense	Annealing temperature, °C	Cycles
S14	GGCAGACCGAGATGAATCCTCA	CAGGTCCAGGGGTCTTGGTCC	65	29
mb1 (CD79a)	GATGCCGGGGATGAATATGAAG	GCTCCCCTAGAGGCAGCGA	60	33
CD19	TCACCGTGGCAACCTGACCATG	GAGACAGCACGTTCCCGTTACTG	65	40
CD10	CTGTGAGAATGATCGCACTCTATG	GATTCCAGTGCATTCATAGTAATC	65	35
TdT	ACACGAATGCAGAAAGCAGGA	AGGCAACCTGAGCTTTTCAAA	60	33
EBF	CTCACTTTGAGAAGCAGCCGC	CATGTCACGTGGGTTTCCCGC	65	32
Pax5	AGCAGGACAGGACATGGAGGA	ATCCTGTTGATGGAACTGACGC	65	32
Pu-1	TGGAAGGGTTTCCCCTCGTC	TGCTGTCCTTCATGTCGCCG	60	33
Gata 3	ACTCCTACATGGACGCGGC	GGTGGATGGACGTCTTGGAG	60	35
Rag-1	GAGAGCAGAGAACACACTTT	CTTTTCAAAGGATCTCACCC	55	32
c-fms	CAGCACCAACAACGCTACCTTC	TCGAGGTTGAGGGTCAGGACTT	65	35

Probe	Sense	Antisense	Annealing temperature, °C	Cycles
S14	GGCAGACCGAGATGAATCCTCA	CAGGTCCAGGGGTCTTGGTCC	65	29
mb1 (CD79a)	GATGCCGGGGATGAATATGAAG	GCTCCCCTAGAGGCAGCGA	60	33
CD19	TCACCGTGGCAACCTGACCATG	GAGACAGCACGTTCCCGTTACTG	65	40
CD10	CTGTGAGAATGATCGCACTCTATG	GATTCCAGTGCATTCATAGTAATC	65	35
TdT	ACACGAATGCAGAAAGCAGGA	AGGCAACCTGAGCTTTTCAAA	60	33
EBF	CTCACTTTGAGAAGCAGCCGC	CATGTCACGTGGGTTTCCCGC	65	32
Pax5	AGCAGGACAGGACATGGAGGA	ATCCTGTTGATGGAACTGACGC	65	32
Pu-1	TGGAAGGGTTTCCCCTCGTC	TGCTGTCCTTCATGTCGCCG	60	33
Gata 3	ACTCCTACATGGACGCGGC	GGTGGATGGACGTCTTGGAG	60	35
Rag-1	GAGAGCAGAGAACACACTTT	CTTTTCAAAGGATCTCACCC	55	32
c-fms	CAGCACCAACAACGCTACCTTC	TCGAGGTTGAGGGTCAGGACTT	65	35