Characteristics of stomatocytic erythrocytes
. | Patient 1 . | Control . | Patient 2 . | Control . |
|---|---|---|---|---|
| Filtration rate, % buffer control | 21 ± 12 | 92 ± 9 | 67 ± 5 | 90 ± 2 |
| Erythrocyte adherence | ||||
| Shear force, dyne/cm2 | ||||
| 20 | 10 (2000) | 7 (2000)* | 9 (1000) | 3 (1000) |
| 30 | 6 (2000) | 4 (2000)* | 5 (1000) | 2 (1000) |
| 55 | 3 (2000) | 1 (2000)* | 2 (1000) | 0 (1000) |
| Thrombospondin adhesion erythrocytes/mm2 | <1 | 9† | 80 | 30 |
| Laminin adhesion erythrocytes/mm2 | <1 | 102† | 180 | 150 |
| Annexin V binding, %‡ | ||||
| Unfractionated erythrocytes | 2.34 + 0.12§ | 0.80 + 0.16 | 2.77 + 0.25∥ | 0.94 + 0.08 |
| Reticulocyte enriched¶ | 2.25 + 0.36# | 0.27 + 0.05 | 2.19 + 0.03** | 0.58 + 0.02 |
| Reticulocyte depleted¶ | 0.83 + 0.09** | 0.08 + 0.01 | 1.71 + 0.16†† | 0.47 + 0.01 |
. | Patient 1 . | Control . | Patient 2 . | Control . |
|---|---|---|---|---|
| Filtration rate, % buffer control | 21 ± 12 | 92 ± 9 | 67 ± 5 | 90 ± 2 |
| Erythrocyte adherence | ||||
| Shear force, dyne/cm2 | ||||
| 20 | 10 (2000) | 7 (2000)* | 9 (1000) | 3 (1000) |
| 30 | 6 (2000) | 4 (2000)* | 5 (1000) | 2 (1000) |
| 55 | 3 (2000) | 1 (2000)* | 2 (1000) | 0 (1000) |
| Thrombospondin adhesion erythrocytes/mm2 | <1 | 9† | 80 | 30 |
| Laminin adhesion erythrocytes/mm2 | <1 | 102† | 180 | 150 |
| Annexin V binding, %‡ | ||||
| Unfractionated erythrocytes | 2.34 + 0.12§ | 0.80 + 0.16 | 2.77 + 0.25∥ | 0.94 + 0.08 |
| Reticulocyte enriched¶ | 2.25 + 0.36# | 0.27 + 0.05 | 2.19 + 0.03** | 0.58 + 0.02 |
| Reticulocyte depleted¶ | 0.83 + 0.09** | 0.08 + 0.01 | 1.71 + 0.16†† | 0.47 + 0.01 |
Values for erythrocyte adherence outside the parentheses represent total adherent cells, and inside the parentheses they represent total RBCs observed.
Sickle cell controls (total adherent cells/total RBCs observed): 20 dyne/cm2, 10.9/1000; 30 dyne/cm2, 6.5/1000; 30 dyne/cm2, 9.1/1000.
Sickle cell controls: thrombospondin, 750 erythrocytes/mm2; laminin, 2500 erythrocytes/mm2.
P = .0002 (unpaired Student t test).
P = .0003.
Manual reticulocyte counts (%). Enriched fraction: patient 1 and control, 24% ± 4% and 1% ± 0.2%; patient 2 and control, 32.3% ± 8.1% and 1.96% ± 0.8%. Depleted fraction: patient 1 and control, 6.5% ± 1.4% and 0%; patient 2 and control, 1.71% ± 0.01% and 0.47% ± 0.01%.
P = .0007.
P = .0001.
P = .0002.