Table 1. D d -ζ cells inhibit the... | American Society of Hematology

Table 1.

D^d-ζ cells inhibit the generation of anti-D^d cytotoxicity in MLC

		P815					EL4
Responder	Stimulator	40:1*	20:1	10:1	40:1	20:1	10:1
FVB pr†	D^d-ζ irr‡	100	100	100	67	31	23
FVB pr	D^d-ζ	34	19	11	100	100	98
D^d-ζ	FVB pr irr	33	28	23	100	100	100
FVB pr	MHC-D^d irr	100	100	99	57	38	25
FVB pr	MHC-D^d	100	100	85	49	37	22

		P815					EL4
Responder	Stimulator	40:1*	20:1	10:1	40:1	20:1	10:1
FVB pr†	D^d-ζ irr‡	100	100	100	67	31	23
FVB pr	D^d-ζ	34	19	11	100	100	98
D^d-ζ	FVB pr irr	33	28	23	100	100	100
FVB pr	MHC-D^d irr	100	100	99	57	38	25
FVB pr	MHC-D^d	100	100	85	49	37	22

Stimulator and responder splenocytes were incubated in MLC at a 1:1 ratio for 5 days, harvested, and tested against ⁵¹Cr-labeled P815 or EL4 target cells. The D^d-ζ cells were obtained from homozygous 130 strain mice.

Chromium release cytotoxicity assay results are listed as the percentage of specific lysis at the indicated effector-to-target cell ratios.

†

Stimulators or responders marked “pr” were primed at least 4 weeks prior to use with an intraperitoneal injection of 20 million splenocytes from an MHC-D^d transgenic mouse.

‡

Stimulators marked “irr” were irradiated at a dose of 2000 rad.

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