Synthesis of cDNA probes for Northern blot hybridization
Target . | GenBank accession no. . | Probe region . | Comment . | Primers . | Transcript size . |
|---|---|---|---|---|---|
| 18S | M10098 | 694-1301 | Structural | 5′-CATTGGAGGGCAAGTCTGG-3′ | 1.9 kb |
| 5′-TTCCTTTAAGTTTCAGCTTTGC-3′ | |||||
| AML-1 | D43968 | 6547-7259 | 3′ UTR | 5′-GGTTTGCTAAATACTGTAGGG-3′ | 6.8 + 7.2 kb |
| 5′-CTTGTCAAACTGTTTATTTGCC-3′ | |||||
| C/EBP-α | Y11525 | 1453-2132 | 3′ UTR | 700-bp EcoRI/HindIII fragment42 | 2.6 kb |
| C/EBP-β | X52560 | 1501-1864 | 3′ UTR | 5′-ATCTATATTTTGCCAACCAACC-3′ | 1.8 kb |
| 5′-AGATTCCCAAAATATACAGACG-3′ | |||||
| C/EBP-δ | S63168 | 953-1335 | 3′ UTR | 5′-CGGGAGAGACTCAGCAACG-3′ | 1.2 kb |
| 5′-GATTTCAAATGCTGCTTTATTC-3′ | |||||
| C/EBP-ϵ | U48865 | 3349-3592 | 3′ UTR + CR | 5′-CCTCCGCAACCTCTTCCG-3′ | 1.4 kb49 |
| 5′-ACAGTGCAACTTTATTCAGCC-3′ | |||||
| C/EBP-γ | U20240 | 569-996 | 3′ UTR + CR | 5′-AAAATCAAATTGCTGACCAAGG-3′ | 1 + 4 kb41 |
| 5′-GTCAGGATTTATTAAGAACATCG-3′ | |||||
| C/EBP-ξ | S40706 | 391-770 | 3′ UTR + CR | 5′-CAAGCACCTCCCAGAGCC-3′ | 0.9 kb |
| 5′-TCATTGGCACTAGTGAGAGG-3′ | |||||
| CASP | L12579 | 1093-1275 | CR | The same probe as for CDP | 3.4 kb59 |
| CDP | M74099 | 1084-1714 | CR | 5′-CCAGGCTGACTATGAAGAGG-3′ | 12 kb59 |
| 5′-GGCGATTTCTGCAGTGTCC-3′ | |||||
| c-fos | K00650 | 2907-3303 | CR | 5′-CTGCCTCTCCTCAATGACC-3′ | 2.6 kb |
| 5′-TGAGCGAGTCAGAGGAAGG-3′ | |||||
| c-jun | J04111 | 2844-3404 | 3′ UTR | 5′-TATTTCTTGTTTGTTTGTTTGGG-3′ | 3.3 kb |
| 5′-AATTCCTGCTTTGAGAATAAGC-3′ | |||||
| CLC | L01664 | 1-598 | CR | Provided by S. Ackerman, University of Illinois, Chicago | 0.9 kb |
| c-myb | M13665 | 2297-2840 | 3′ UTR | 5′-AAGGTACATTTATTGTACCAAACC-3′ | 2.5 + 3.8 kb76 |
| 5′-CAGTTGCAAACACAGGATCC-3′ | |||||
| Elf-1 | M82882 | 3013-3526 | 3′-end | 5′-GTTATGGGTCAAGATCTGCC-3′ | 3.6 kb |
| 5′-TACAAAAATGTTTAGAGGATTCC-3′ | |||||
| FMLP-R | M60626 | 31-551 | CR | 5′-CCAGGAGCAGACAAGATGG-3′ | 1.9 kb |
| 5′-CCAGGTACTGTAGTCACACG-3′ | |||||
| GATA-1 | X17254 | 175-677 | CR | 5′-TGCTCTGGTGTCCTCCACACC-3′ | 1.5 kb |
| 5′-TGGGAGAGGAATAGGCTGCT-3′ | |||||
| Glycophorin A | X08054 | 373-898 | 3′ UTR + CR | 5′-CGATCCTCTTAATTTCTTACGG-3′ | 0.7, 1.0, 1.7 + 2.6 kb77 |
| 5′-CTTCATCTAGGTATTTCAAGCC-3′ | |||||
| Lactoferrin | U07643 | 1864-2018 | CR | 5′-AGACTTTGCGCTGCTGTGCC-3′ | 2.4 kb |
| 5′-CAGTCAGATCCATTTCTCCC-3′ | |||||
| MPO | X04876 | 1683-1868 | CR | 5′-CTTCATGTTCCGCCTGGACA-3′ | 3.2 kb |
| 5′-CGGATCTCATCCACTGCAAT-3′ | |||||
| PU.1 | X52056 | 213-758 | CR | 5′-CCTGGGGAGACAGGCAGC-3′ | 1.4 kb |
| 5′-GAGTCCTGGAGGGAGGCG-3′ | |||||
| β-actin | X00351 | 1028-1719 | 3′ UTR + CR | 5′-CATTGCTCCTCCTGAGCGC-3′ | 1.8 kb |
| 5′-AGGTAAGCCCTGGCTGCC-3′ |
Target . | GenBank accession no. . | Probe region . | Comment . | Primers . | Transcript size . |
|---|---|---|---|---|---|
| 18S | M10098 | 694-1301 | Structural | 5′-CATTGGAGGGCAAGTCTGG-3′ | 1.9 kb |
| 5′-TTCCTTTAAGTTTCAGCTTTGC-3′ | |||||
| AML-1 | D43968 | 6547-7259 | 3′ UTR | 5′-GGTTTGCTAAATACTGTAGGG-3′ | 6.8 + 7.2 kb |
| 5′-CTTGTCAAACTGTTTATTTGCC-3′ | |||||
| C/EBP-α | Y11525 | 1453-2132 | 3′ UTR | 700-bp EcoRI/HindIII fragment42 | 2.6 kb |
| C/EBP-β | X52560 | 1501-1864 | 3′ UTR | 5′-ATCTATATTTTGCCAACCAACC-3′ | 1.8 kb |
| 5′-AGATTCCCAAAATATACAGACG-3′ | |||||
| C/EBP-δ | S63168 | 953-1335 | 3′ UTR | 5′-CGGGAGAGACTCAGCAACG-3′ | 1.2 kb |
| 5′-GATTTCAAATGCTGCTTTATTC-3′ | |||||
| C/EBP-ϵ | U48865 | 3349-3592 | 3′ UTR + CR | 5′-CCTCCGCAACCTCTTCCG-3′ | 1.4 kb49 |
| 5′-ACAGTGCAACTTTATTCAGCC-3′ | |||||
| C/EBP-γ | U20240 | 569-996 | 3′ UTR + CR | 5′-AAAATCAAATTGCTGACCAAGG-3′ | 1 + 4 kb41 |
| 5′-GTCAGGATTTATTAAGAACATCG-3′ | |||||
| C/EBP-ξ | S40706 | 391-770 | 3′ UTR + CR | 5′-CAAGCACCTCCCAGAGCC-3′ | 0.9 kb |
| 5′-TCATTGGCACTAGTGAGAGG-3′ | |||||
| CASP | L12579 | 1093-1275 | CR | The same probe as for CDP | 3.4 kb59 |
| CDP | M74099 | 1084-1714 | CR | 5′-CCAGGCTGACTATGAAGAGG-3′ | 12 kb59 |
| 5′-GGCGATTTCTGCAGTGTCC-3′ | |||||
| c-fos | K00650 | 2907-3303 | CR | 5′-CTGCCTCTCCTCAATGACC-3′ | 2.6 kb |
| 5′-TGAGCGAGTCAGAGGAAGG-3′ | |||||
| c-jun | J04111 | 2844-3404 | 3′ UTR | 5′-TATTTCTTGTTTGTTTGTTTGGG-3′ | 3.3 kb |
| 5′-AATTCCTGCTTTGAGAATAAGC-3′ | |||||
| CLC | L01664 | 1-598 | CR | Provided by S. Ackerman, University of Illinois, Chicago | 0.9 kb |
| c-myb | M13665 | 2297-2840 | 3′ UTR | 5′-AAGGTACATTTATTGTACCAAACC-3′ | 2.5 + 3.8 kb76 |
| 5′-CAGTTGCAAACACAGGATCC-3′ | |||||
| Elf-1 | M82882 | 3013-3526 | 3′-end | 5′-GTTATGGGTCAAGATCTGCC-3′ | 3.6 kb |
| 5′-TACAAAAATGTTTAGAGGATTCC-3′ | |||||
| FMLP-R | M60626 | 31-551 | CR | 5′-CCAGGAGCAGACAAGATGG-3′ | 1.9 kb |
| 5′-CCAGGTACTGTAGTCACACG-3′ | |||||
| GATA-1 | X17254 | 175-677 | CR | 5′-TGCTCTGGTGTCCTCCACACC-3′ | 1.5 kb |
| 5′-TGGGAGAGGAATAGGCTGCT-3′ | |||||
| Glycophorin A | X08054 | 373-898 | 3′ UTR + CR | 5′-CGATCCTCTTAATTTCTTACGG-3′ | 0.7, 1.0, 1.7 + 2.6 kb77 |
| 5′-CTTCATCTAGGTATTTCAAGCC-3′ | |||||
| Lactoferrin | U07643 | 1864-2018 | CR | 5′-AGACTTTGCGCTGCTGTGCC-3′ | 2.4 kb |
| 5′-CAGTCAGATCCATTTCTCCC-3′ | |||||
| MPO | X04876 | 1683-1868 | CR | 5′-CTTCATGTTCCGCCTGGACA-3′ | 3.2 kb |
| 5′-CGGATCTCATCCACTGCAAT-3′ | |||||
| PU.1 | X52056 | 213-758 | CR | 5′-CCTGGGGAGACAGGCAGC-3′ | 1.4 kb |
| 5′-GAGTCCTGGAGGGAGGCG-3′ | |||||
| β-actin | X00351 | 1028-1719 | 3′ UTR + CR | 5′-CATTGCTCCTCCTGAGCGC-3′ | 1.8 kb |
| 5′-AGGTAAGCCCTGGCTGCC-3′ |
Transcript sizes were found in the GenBank database or, where indicated, in publications.
CASP indicates cut alternatively spliced protein; CLC, Charcot-Leyden crystal; fMLP-R, formyl-Met-Leu-Phe receptor; MPO, myeloperoxidase; UTR, untranslated region; and CR, coding region.