Flow cytometric determination of contaminating cells in the neutrophil cell preparations
Cell type* . | MBs/PMs, % . | MBs/PMs depleted, % . | MCs/MMs, % . | MCs/MMs depleted, % . | BCs/SCs, % . | BCs/SCs depleted, % . | PMNs, % . | PMNs depleted, % . |
|---|---|---|---|---|---|---|---|---|
| B cells | 6.5 (3.8-8.1) | 0.53 (0-1.2) | 1.3 (0-3.4) | 0.11 (0-0.4) | 0.17 (0-0.5) | 0.19 (0-1.1) | ND | ND |
| T cells | 20 (13-28) | 1.3 (0-3.3) | 5.6 (0-10) | 0.09 (0-0.5) | 0.46 (0-1.0) | 0.02 (0-0.1) | ND | ND |
| NK cells | 6.3 (1.6-10) | 3.8 (0-10) | 0.43 (0-1.6) | 0.23 (0-1.1) | 0.01 (0-0.3) | 0.14 (0-0.9) | ND | ND |
| Erythroid cells | 12 (6.8-21) | 0.01 (0-0.06) | 12 (8.4-13) | 0.28 (0-1.1) | 5.9 (0-17) | 0.34 (0-0.8) | ND | ND |
| Monocytes | 14 (8.6-19) | 1.3 (0.6-2.2) | 1.6 (0.1-2.5) | 0.91 (0.5-2.3) | 1.3 (0.5-1.9) | 0.40 (0-1.4) | ND | ND |
| Megakaryocytes | 9.3 (4.5-21) | 0.74 (0-2.4) | 1.4 (0.6-2.7) | 0.39 (0-0.8) | 2.1 (0-3.9) | 0.52 (0-1.3) | ND | ND |
| Eosinophils | ND | ND | ND | ND | 5.7 (3.1-8.7) | 0.57 (0.1-2.0) | 4.0 (2.6-6.7) | 0.69 (0-1.5) |
| Total contamination | 68 (55-75) | 7.7 (3.2-13) | 22 (11-30) | 2.0 (1.0-3.4) | 16 (9.5-24) | 2.2 (0.4-6.6) | 4.0 (2.6-6.7) | 0.7 (0-1.5) |
Cell type* . | MBs/PMs, % . | MBs/PMs depleted, % . | MCs/MMs, % . | MCs/MMs depleted, % . | BCs/SCs, % . | BCs/SCs depleted, % . | PMNs, % . | PMNs depleted, % . |
|---|---|---|---|---|---|---|---|---|
| B cells | 6.5 (3.8-8.1) | 0.53 (0-1.2) | 1.3 (0-3.4) | 0.11 (0-0.4) | 0.17 (0-0.5) | 0.19 (0-1.1) | ND | ND |
| T cells | 20 (13-28) | 1.3 (0-3.3) | 5.6 (0-10) | 0.09 (0-0.5) | 0.46 (0-1.0) | 0.02 (0-0.1) | ND | ND |
| NK cells | 6.3 (1.6-10) | 3.8 (0-10) | 0.43 (0-1.6) | 0.23 (0-1.1) | 0.01 (0-0.3) | 0.14 (0-0.9) | ND | ND |
| Erythroid cells | 12 (6.8-21) | 0.01 (0-0.06) | 12 (8.4-13) | 0.28 (0-1.1) | 5.9 (0-17) | 0.34 (0-0.8) | ND | ND |
| Monocytes | 14 (8.6-19) | 1.3 (0.6-2.2) | 1.6 (0.1-2.5) | 0.91 (0.5-2.3) | 1.3 (0.5-1.9) | 0.40 (0-1.4) | ND | ND |
| Megakaryocytes | 9.3 (4.5-21) | 0.74 (0-2.4) | 1.4 (0.6-2.7) | 0.39 (0-0.8) | 2.1 (0-3.9) | 0.52 (0-1.3) | ND | ND |
| Eosinophils | ND | ND | ND | ND | 5.7 (3.1-8.7) | 0.57 (0.1-2.0) | 4.0 (2.6-6.7) | 0.69 (0-1.5) |
| Total contamination | 68 (55-75) | 7.7 (3.2-13) | 22 (11-30) | 2.0 (1.0-3.4) | 16 (9.5-24) | 2.2 (0.4-6.6) | 4.0 (2.6-6.7) | 0.7 (0-1.5) |
Data are presented as mean (variation).
ND indicates not determined.
Cell types include precursors.