Table 3.

PCR antisense primers used to sequence kininogen intron 9


Location

Antisense primers*

Position on cDNA/DNA
Exon 10   5′-ATGGCCCCAGTCATGTCTAC-3′   165-184  
Exon 10   5′-AAACCTGGAGGCCTTTTCAT-3′   20-39  
Intron 9   5′-tctcagcactttgggagacc-3′   1822-1841  
Intron 9   5′-aaaattagctgggcatggtg-3′   1721-1740  
Intron 9
 
5′-actttgttgtgctgaagcac-3′
 
1297-1316
 

Location

Antisense primers*

Position on cDNA/DNA
Exon 10   5′-ATGGCCCCAGTCATGTCTAC-3′   165-184  
Exon 10   5′-AAACCTGGAGGCCTTTTCAT-3′   20-39  
Intron 9   5′-tctcagcactttgggagacc-3′   1822-1841  
Intron 9   5′-aaaattagctgggcatggtg-3′   1721-1740  
Intron 9
 
5′-actttgttgtgctgaagcac-3′
 
1297-1316
 
*

The sequences shown represent the antisense oligonucleotides prepared from the nucleotides at the position presented.

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