Constructs were initially prepared in vector pSP72 and subcloned into pSEAP. basic. The number indicates the 5′ most hEPCR nucleotide contained within the construct.

For constructs generated using restriction enzymes, vector pSP72.-2336 was digested with BamHI and the enzyme listed. The fragment containing vector and hEPCR sequence was blunt ended, and the vector was recircularized. Constructs generated by PCR used the primers listed and the same downstream primer.